五味子乙素对UVA损伤组织工程皮肤保护作用的研究.docVIP

五味子乙素对UVA损伤组织工程皮肤保护作用的研究 　　[摘要]目的：探讨五味子乙素（Sch B）对长波紫外线（UVA）接种人永生化角质形成细胞（Ha Cat细胞）和成纤维细胞（FB细胞）的组织工程皮肤模型损伤后的保护作用及其可能的作用机制。方法：用5J/cm2的UVA照射组织工程皮肤模型，给予不同浓度的Sch B（0.01、0.1、1、10、100μmol/L）处理UVA损伤后的组织工程皮肤模型，检测细胞超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-PX）的活性，乳酸脱氢酶（LDH）及一氧化氮（NO）含量。结果：UVA照射组织工程皮肤模型后，SOD、GSH-PX活性降低，LDH及NO含量升高，给予不同浓度的Sch B对SOD、GSH-PX活性、LDH及NO含量，有不同的影响，实验结果表明以0.01、0.1μmol/L Sch B的保护做用最强。结论：0.01、0.1μmol/L Sch B减轻UVA对组织工程皮肤模型损伤的效果最佳。 　　[关键词]五味子乙素；紫外线；组织工程皮肤 　　[中图分类号]R3181[文献标识码]A[文章编号]1008-6455（2014）06-0457-04 　　 　　Research on effects of Sanhedrin B protective effect on the long wave ultraviolet damage tissue engineering skin 　　ZHOU Yu-jie1,2,3,LI Rui-xin2,SUN Kai2,NIAN Zheng-hao2,MA Dong-dong1,LU Tao3 　　（1.Hebe United University,Tangshan 063009,Hebe,China;2.Institute of Medical Equipment,Academy of Military and Medical Sciences,Tianjin 300161,China；3.The Affiliated Hospital of logistical College of the Chinese Peoples Armed Police Force,Tianjin 300162,China） 　　 　　Abstract:ObjectiveThe aim to explore the effect of Sanhedrin B (Sch B) on tissue engineering skin injury caused by the long wave ultraviolet (UVA) and its possible Mechanism.MethodsTissue engineering skin was irradiated with a dose of 5 J/cm2 UVA, treated with different concentrations of Sch B（0.01,0.1,1,10,100μmol/L） treatment after UVA damage of tissue engineering skin,the detection cell superoxide diastase (SOD) and glutathione peroxidase (GSH-PX) activity,lactate dehydrogenase (LDH) and nitric oxide (NO) content. ResultsAfter tissue engineering skin was irradiated by UVA,the result of which reduced the SOD,GSH-PX activity,increased LDH and NO content,treated with different concentrations of Sch B,which couldaffect the SOD,GSH,LDH,NO activity,to improve the survival rate tissue engineering skin, especially with the protection of the 0.01,0.1μmol/L Sch B do with strongest.Conclusion0.01,0.1μmol/L Sch B can be a cert ain extent and reduce the UVA damage of tissue engineering skin. 　　Key words:Sanhedrin