余甘子叶药材的HPLC指纹图谱研究.docVIP

余甘子叶药材的HPLC指纹图谱研究 　　[摘要] 目的 建立余甘子叶的指纹图谱。 方法 采用高效液相色谱法测定，乙腈-0.1%磷酸溶液梯度洗脱，流速1.0 ml/min，柱温25℃，紫外检测波长218 nm。 结果 余甘子叶中有15个共有峰被确定为指纹峰，采用《中药色谱指纹图谱相似度评价系统A版》计算机软件评价10批余甘子叶指纹图谱的相似性，其结果均0.9。 结论 该方法简便，稳定，精密度高，重现性好，可用于余甘子叶的鉴别，作为质量控制方法。 　　[关键词] 余甘子叶；高效液相色谱法；指纹图谱 　　[中图分类号] R284.1[文献标识码] A[文章编号] 1674-4721（2014）06（b）-0011-04 　　 　　Study on HPLC fingerprint analysis of Phyllanthus emblica L. 　　MAO Jian1 LIU Zhen-jie2▲ 　　1.Department of Clinical medicine,People′s Hospital of Fuchuan County in Hezhou City of Guangxi Zhuang Autonomous Region,Fuchuan 542700,China;2.Conservation Center,Guangxi Institute of Medicinal Plant,Guangxi Zhuang Autonomous Region,Nanning 530023,China 　　[Abstract] Objective To establish a method of fingerprint analysis on Phyllanthus emblica L. by HPLC. Methods The chromatographic finger-prints were obtained by HPLC.The detective wave-length was set at 218 nm;the flow rate was 1.0 ml/min and the column temperature was maintained at 25℃. Results 15 co-possessing peaks were selected as the fingerprint peaks of Phyllanthus emblica L. and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of Phyllanthus emblica L. and the standard fingerprint. Conclusion The method with good reproducibility is simple and accurate,it can be used as an effective quality control method for Phyllanthus emblica L. 　　[Key words] Phyllanthus emblica L.;High performance of liquid chromatography;Fingerprint 　　余甘子叶（Phyllanthus emblica L.）系大戟科（Euphorbiaceae）叶下珠属（Phyllanthus）余甘子Phyllanthus emblical L.落叶小乔木或灌木的叶[1]，余甘子树在我国资源丰富，收载于1978年版《藏药标准》、1974年出版的《云南省药品标准》和多版《中华人民共和国药典》，别名有橄榄、滇橄榄、油甘子、山油甘、庵摩勒、牛甘子、喉甘子、杨甘等,余甘子果果实生食酸甜酥脆，初食味酸涩，后回味甘甜爽口，故名余甘[2]。余甘子味甘、酸、涩、性凉，具有化痰止咳，健胃消食，清热生津，保肝解毒等功效[3]。主治感冒发热、咳嗽、咽喉痛、白喉、烦热口干、消化不良、腹痛、慢性肝炎、高血压、肥胖症、高脂血症、湿热性水肿、尿频、血病、赤巴病、培根病、肝病、心脏病，以治疗血热血瘀引起的血病为长[4]。近年研究表明余甘子具有抗菌[5]、抗肿瘤[6]、抗氧化[7]、抗动脉粥样硬化[8-9]、抗乙型肝炎病毒[10]、保肝[11]、增强免疫[12-13]等作用。余甘子有多种化学成分，药理活性研究主要集中在多酚类成分上，槲皮素、没食子酸为其有效成分[14-15]。本实验对余甘子叶进行指纹图谱研究