第二讲摘要写作.ppt

A total of 228 markers were scored/obtained/gained from the 21 primers, which were screened out from 90 primers and demonstrated highly reproducible, clear and polymorphic bands. Among the obtained markers, 199 were polymorphic, accounting for 87.28% of the total. As analyzed/computed by NTSYS2.01, the genetic similarity among the accessions ranged from 0.49 to 0.90, reflecting/indicating/mirroring/suggesting the high genetic diversity of cherry germplasms in Guizhou Province. The UPGMA dendrogram demonstrated/indicated that the accessions from Bijie, Zunyi and Qiannan areas were more genetically diversified. In particular the accessions from Bijie exhibited the highest degree of diversity in fruit quality, which may be used as elite materials for further genetic improvement in this species. Effects of the various/diverse/different concentrations of either mannitol, PP333, or ABA on slow growth of in vitro Pinellia ternate were investigated, and the somaclonal variation was further assessed/detected using ISSR marker in the current work. Compared with the normal subculturing plantlet/control, the morphological traits, as well as proliferation and rooting ability of regeneration plant were not significantly different. The optimal concentration of mannitol, PP333 and ABA were 2%-4%, 2.0 mg.L-1 and 2.0 - 4.0 mg.L-1, respectively, for long-time preservation of Pinellia ternate. As detected by ISSR molecular, no polymorphic/aberrant marker was scored/ observed/investigated/ obtained from the regenerated plantlets conserved on medium with the addition of 2% mannitol and 2.0 mg.L-1 PP333. However/Conversely, a new marker and a absent mark were observed from that conserved on medium supplemented with 2.0 mg.L-1 ABA, and the locus or individual variation rate was 1.6% or 30%, respectively. Therefore, ABA was not suitable for in vitro conservation of Pinellia germplasm. Rather, it might be used to generate new mutant, which demonstrated highly a promising role