DNA测序发展及其展望要素.pdf

TECHNOLOGY e-Science 技术 DNA测序技术发展及其展望 1,2 1 孙海汐 王秀杰 1.中国科学院遗传与发育生物学研究所植物基因组学国家重点实验室，北京100101 2.中国科学院研究生院，北京100049 摘 要：DNA测序技术是现代生物学研究中重要的手段之一。自从1977年第一代测序 技术问世以来，经过三十几年的努力，DNA测序技术已经取得了很大的发展， 在第一代和第二代测序技术的基础上，以单分子测序为特点的第三代测序技 术已经诞生。本文回顾了每一代测序技术的原理和特点，并对测序技术的发 展趋势和它在生物学研究中的应用做了展望，以期更好地帮助人们理解测序 技术在生命科学研究中的重要作用。 关键词： 第一代测序技术；第二代测序技术；第三代测序技术；基因芯片技术 The Development and Future Perspectives of DNA Sequencing Technology Sun Haixi1,2 ,Wang Xiujie1 1 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Beijing 100101,China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China Abstract: DNA sequencing technology has played important roles in modern biological research. The first generation of sequencing technology appeared in 1977. During the past decade, the so-called next generation sequencing technologies had emerged and caused a revolution in biological research. Recently, the third generation sequencing technology, which is able to determine the base composition of single DNA molecules, has joined the race. The mechanisms and features of each generation of sequencing technology and their future perspectives will be discussed here. Keywords: first generation sequencing technology; second generation sequencing technology; third generation sequencing