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Review of Phlebotomy and Laboratory Requirements放血和实验室要求审查
Review of Phlebotomy and Laboratory Requirements Janet Vincent, MS, SBB(ASCP) Education Coordinator Department of Pathology Phlebotomy! One of the most often remembered experiences One of the least trained fields in all of medicine One question on the Press Gainey survey that relates to the Laboratory. What is the most important step in drawing a blood sample? Universal Precautions Not hurting the patient Wearing gloves Getting the right tube of blood Something else? The most important step in drawing a blood sample: Patient Identification! Second? Labeling the specimen CORRECTLY Steps in the process Introduce yourself and explain procedure Patient Identification Tie the tourniquet Search for a vein Prepare the site Draw the correct tubes, in the correct order Invert the tubes Remove the tourniquet and needle. Apply pressure to site The supplies ID Patient, look for a vein, cleanse site Anchor vein, perform venipuncture Angle of draw is 15-300 Remove Tourniquet Label the tube!!!! Things you should not do! Do not leave the Tourniquet on longer than 2 minutes. Do not leave the tourniquet on when you are removing the needle Do not perform the venipuncture while the alcohol is still wet Do not leave the tube on the back of the needle when removing the needle from the arm Do not enter the vein slowly. To prevent a hematoma: Puncture only the uppermost wall of the vein Remove the tourniquet before removing the needle Use the major superficial veins Make sure the needle fully penetrates the upper most wall of the vein. (Partial penetration may allow blood to leak into the soft tissue surrounding the vein by way of the needle bevel) Apply pressure to the venipuncture site Hemotoma To prevent hemolysis Mix tubes with anticoagulant additives gently 5-10 times Avoid drawing blood from a hematoma When using a needle and syringe, draw the plunger back gently to avoid frothing of the sample Make sure the venipucture site is dry Avoid a probing, traumatic venipucture Hemocon
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