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Early and delayed afterdepolarizations in rabbit heart Purkinje cells viewed by confocal microscopy.pdf

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Early and delayed afterdepolarizations in rabbit heart Purkinje cells viewed by confocal microscopy.pdf

Research Cell Calcium (2001) 29(5), 289–297 ? 2001 Harcourt Publishers Ltd doi: 10.1054/ceca.2000.0192, available online at on Early and delayed afterdepolarizations in rabbit heart Purkinje cells viewed by confocal microscopy J. M. Cordeiro, J. H. B. Bridge, K. W. Spitzer Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah School of Medicine, Salt Lake City, Utah, USA Summary We investigated action potentials and Ca2; transients in rabbit Purkinje myocytes using whole cell patch clamp recordings and a confocal microscope. Purkinje cells were loaded with 5 ?M Fluo-3/AM for 30 min. Action potentials were elicited by application of a stimulus delivered through the recording pipettes. When Purkinje cells were stimulated in 2.0 mM Ca2;, transverse XT line scans revealed a symmetrical ‘U’-shaped Ca2; transient demonstrating that the transient was initiated at the cell periphery. When Purkinje cells were superfused with 1 ?M isoprenaline, both early and delayed afterdepolarizations were induced. XT line scans of cells exhibiting early afterdepolarizations showed a second symmetrical ‘U’-shaped transient. This Ca2; transient was initiated at the cell periphery suggesting reactivation of the Ca2; current. In contrast, in Purkinje cells exhibiting delayed afterdepolarizations and a corresponding transient inward current, XT line scans revealed a heterogenous rise in Ca2; at both peripheral and central regions of the cell. Immunofluorescence staining of Purkinje cells with an antibody to ryanodine receptors (RyRs) revealed that RyRs are located at regularly spaced intervals throughout the interior of Purkinje cells. These results suggest that, although RyRs are located throughout Purkinje cells, only peripheral RyRs are activated to produce transients, sparks and early afterdepolarizations. During delayed afterdepolarizations, we observed a heterogenous rise in Ca2; at both peripheral and central regions of the cell as well as large centra