Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts.pdfVIP

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Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts.pdf

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Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts.pdf

Contact Lens and Anterior Eye 39 (2016) 239–243 Contents lists available at ScienceDirect Contact Lens and Anterior Eye journal homepage: /locate/clae Short communication Targeting cyst wall is an effective strategy in improving the ef?cacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts Farhat Abjania,1, Naveed Ahmed Khanb,1,*, Farzana Abubakar Yousufa, Ruqaiyyah Siddiquib a Department of Biological and Biomedical Sciences, Aga Khan University, Pakistan b Department of Biological Sciences, Faculty of Science and Technology, Sunway University, Malaysia ARTICLE INFO Article history: Received 7 September 2015 Received in revised form 29 October 2015 Accepted 24 November 2015 Keywords: Keratitis Contact lens Disinfecting solution Cellulase ABSTRACT Acanthamoeba cysts are highly resistant to contact lens disinfecting solutions. Acanthamoeba cyst wall is partially made of 1,4 b-glucan (i.e., cellulose) and other complex polysaccharides making it a hardy shell that protects the resident amoeba. Here, we hypothesize that targeting the cyst wall structure in addition to antiamoebic compound would improve the ef?cacy of marketed contact lens disinfecting solutions. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the ?ndings revealed that combination of both agents abolished viability of Acanthamoeba castellanii cysts and trophozoites. When tested alone, none of the agents nor contact lens disinfecting solutions completely destroyed A. castellanii cysts and trophozoites. The absence of cyst wall-degrading enzymes in marketed contact lens disinfecting solutions render them ineffective against Acanthamoeba cysts. It is concluded that the addition of cyst wall degrading molecules in contact lens disinfecting solutions will enhance their ef?cacy in decreasing the incidence of Acanthamoeba effectively. ? 2015 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved. 1