拒霜叶中总黄酮的提取工艺研究.pdf

拒霜叶中总黄酮的提取工艺研究 樊俊红 安彩贤 (西安交通大学药学院 陕西 西安 710061 摘要 目的 研究拒霜叶中总黄酮的最佳提取工艺 方法 采用水煎醇沉法提 取拒霜叶中的总黄酮 并用 L9 34 正交实验法对其提取工艺进行优选 结果 最佳工艺条件为 16 倍量水煎煮 3 次 每次 0.5h 然后浓缩至相对密度为 1.15 60 加乙醇使含醇量达 60% 冷藏 24h 结论 此法操作简便 适合大工业生 产 关键词 拒霜叶; 芦丁; 总黄酮; 正交实验 Study on Extraction Process of Leaves of Hibiscus Mutabilis L. FANJUN hong, ANCai xian (College of Pharmacy,Xi’an Jiaotong University,Xi’an,710061,China) Abstract: Objective: To study the optimum extraction condition of flavonoids from the leaves of Hibiscus Mutabilis L.. Methods: The decoction and alcohol deposit method was used to extract flavonoids from the leaves of Hibiscus Mutabilis L.. The orthogonal design L9 34 was used to study the optimum extraction condition. Results: The optimum extraction process was as follows extracted with 16 times the amount of the solvent volume water for 3 times and with each time for half of hour. Then the liquids were condensed until the relative density was 1.15 60 and the alcohol added made the alcohol concentration be 60% in the liquids. Finally the liquids were disposed 24 hours under the cold storage. Conclusion: The method was operated simply and fitted to produce by the industry. Keywords: leaves of Hibiscus Mutabilis L.; rutinum; flavonoid; orthogonal design 拒霜叶为锦葵科 malvaceae 植物木芙蓉 Hibiscus Mutabilis L. 的干燥叶 其味辛 平 无毒 具有凉血 解毒 消肿 止痛等功效 据文献报道 拒霜叶 主要含黄酮苷 酚类 氨基酸 还原糖等 其叶的煎剂对金黄色葡萄球菌 伤寒 杆菌等均有抑制作用 还具有明显的抗炎活性[1] 为探索最佳提取工艺 保证有 效成分的提取率且适合大生产 实验以主要成分总黄酮含量为指标 用正交实验 设计优选出最佳提取工艺 1 仪器与试药 SP-2000UV 型可见-紫外分光光度计 上海光谱仪器有限公司 旋转蒸发 器 RE-52 上海亚荣生化仪器厂 拒