烟酰胺辅酶NADH多活性中心模型物合成及电化学性质+.pdf

烟酰胺辅酶(NADH)多活性中心模型物合成及电化学性质 朱晓晴 张爱芹 程津培 (南开大学化学学院，天津 300071，中国) 摘要：生物活性分子的化学模拟是揭示生物活性分子生物功能背后化学奥秘的一个十分有效研究方法。烟酰 胺辅酶（NADH）是生命体内最重要的氧化还原辅酶之一，担负着转移负氢离子和电子的职能，在生物体内的 新陈代谢和氧化还原过程中起着不可或缺的作用。烟酰胺辅酶(NADH)氧化还原反应特性和机理的研究是当今 生物化学研究领域一个热点课题。本文根据烟酰胺辅酶(NADH)的结构特点设计合成了若干个具有多活性中心 的烟酰胺辅酶新型模型物并研究了它们的电化学性质。在模型物氧化还原电位测定中，我们有趣地发现具有 两个中心的烟酰胺辅酶模型物的电位图均有两个氧化峰，而且当模型物中两个活性中心相邻时，两个氧化峰 的裂分比其他的要大得多，原因可能是当模型物中两个活性中心处于苯环邻位时，两个活性中心可以共享同 一个正电荷。 关键词：辅酶(NAD(P)H) 电子转移 多活性中心 Synthesis of a Novel NADH Model with Multi-centers and Study on the Redox Properties Xiao-Qing Zhu, Ai-Qin Zhang and Jin-Pei Cheng (College of Chemistry, Nankai University，Tianjin 300071，China) Abstract: The chemical simulation of biological active molecules is one of the most useful research methods to uncover chemical nature of biological active molecules related to their biological function. NADH is one of the most important redox coenzyme, and is responsible to transfer a hydride ion or an electron to the suitable substrates during the metabolism and redox processes in organism. Displaying the nature and mechanism of NADH redox process in vivo is one of the most hotspots in the contemporary biochemical researches. In this paper, we wish to report the synthesis and redox properties of novel NADH models with multi-centers[1]. From the determination of the redox potentials it is interesting to find that there were two oxidation peaks for one model molecule with two active centers, and the difference of the potential values of the two peaks is much larger than that of the other NADH models with multi-centers. The main reason could be that the two active centers of the NADH models can share one positive charge when the two active centers located at the ortho–positions of benzene ring for each