12周下坡跑对去卵巢大鼠股骨骨髓脂肪组织容量和微血管密度的影响.pdfVIP

ES噩噩噩言矗雪融fai圈 2014年第29卷第3 期 lournal ofTUS VOl.29 NO .3 2014 目噩噩圆 备忘句 ·研究报道 Short Comunications 12 周下坡跑对去卵巢大鼠股骨骨髓脂肪组织容量和微血管密度 的影响 江大雷1 ， 2，刘海涛l 摘要 日的:探讨运动对去卵巢大鼠骨髓脂肪组织容量及微血管密度的影响。方法:15 只9周龄雌性SD大鼠随机分为3 组:假手术组(SHAM) 、去卵 巢组(OVX) 、去卵巢+运动组(OVX+EX) ，每组5 只， OVX组和OVX+EX组行去卵巢手术，SHAM组只切除卵巢周围少量脂肪组织。 OVX+EX大鼠进 行12 周跑台下坡跑运动，跑速为18 m1min ，每天运动时间为45 min ，每周5 夭。 12 周后，取右侧股骨进行骨密度测定，取左侧股骨远侧干儒端制成石 蜡标本，苏木素-伊红染色(HE)用于观察骨髓病理学改变，计算骨髓脂肪组织容量百分比，采用免疫组织化学技术，CD34抗原标记血管内皮细胞，计 算骨髓微血管密度(MVD)。结果:与SHAM组相比，OVX组大鼠骨密度显著下降(P0.05) ， 提示骨质疏松造模成功;股骨干前端骨髓脂肪组织容量 百分比显著增高(PO.Ol) ， 骨髓MVD显著降低(PO.01);OVX+EX组大鼠骨密度、骨髓MVD均高于OVX组(P0， 05) ， 骨髓脂肪组织容量百分比显著 低于OVX组(P0.05) 。结论: 12 周下坡跑运动可以有效抑制大鼠去卵巢后骨密度的下降，降低骨髓脂肪组织容量，提高骨髓微血管密度。 关键词 下坡跑;骨质疏松;骨髓微血管密度;骨髓脂肪组织 中图分类号:G 804.5 文献标志码:A 文章编号:1005一∞∞(2014)03-268-04 EtTect of 12-Week Downhill running on Femoral Bone Marrow Adipose Tissue Volume and Microvascular Den- sity in Ovariectomized Rats 1 2 1 RANG Dalei ? ? LIU Haitao (l.School of Graduate. Beijing Sports University. Beijing 100084. China; 2.Dept. of PE , Xuchang University , Xuchang 461000 , China) Abstract Objective: TO investigate the effect Of exercise On bOne marrOw adipO se ti随时 vOlume and microvascular density in OvariectOmized rats. MethOds: fiι teen 9-week-Old female SD rats were randOmly divided intO 3 grOups: sham OperatiOn grOup (SHAM) , OvariectOmized grOup (OVX) , dOwnhill running grOup (OVX+EX) , 5 rats in each grOup. The rats Of OVX grOup and OVX+EX grOup underwent OvariectOmy , hOwever Only a small amOunt Of adipO se tissue around the Ovaηwas remOved in SHAM grOup. The rats Of OVX+EX grOup were running dOwnhill On the treadmill fOr 12 weeks , the running speed was 18m1min fOr 45 minutes each day , 5 days per week. After 12 weeks , bOne mineral de