用固相萃取GCMS法检验人体尿液中的曲马多.pdfVIP

用固相萃取GC/MS法检验人体尿液中的曲马多 谢增瑞 河南省郑州市公安局刑科所 郑州450004 摘要 目的 建立固相萃取和 GC/MS联用技术相结合检验人体尿液中曲马多的方法 方 法 用GDX403作固相萃取吸附剂吸附尿液中的曲马多 在初始柱温为 100保持 1min 后 以 20/min升至260保持2min的情况下 用JW 30m0.25mm0.25um DB-5/MS 柱进行 GC/MS分析 结果 当吸附树脂用量在 0.8克以上时 曲马多的回收率在 82.0%-88.0%之间 进样量为2ng时的信噪比 s/n为86:1结论 该方法操作简单 灵敏 回收率高 且不乳化 关键词 固相萃取 GC/MS法 曲马多 烯丙异丙巴比妥 (SKF) 525A determination of the tramal in human urine by coupling solid -phase extraction with gas chromatography-mass spectrometry XIE Zeng-rui (Zhengzhou Institue of Forensic Science zhengzhou 450004) Abstract Objective: To establish a method for the determination of the tramal in human urine by coupling solid -phase extraction with gas chromatography-mass spectrometry. Methods: The solid -phase extraction was used for extracting tramal in human urine and the 0 GC/MS was applied for analysis when the initial temperature of column was kept at 100 C for 0 0 1 min, then raiser to 260 C at rate of 20 C/min and kept for 2 min. Results: When the weight of absorbent was above 0.8g, the recovery rate of tramal in human urine was 82.0%-88.0% by this method. In addition, The ratio of signal to noise(s/n) of tramal was 86:1 as the sample velue was 2ng. Conclusions: The method is simple in operation, seneitive, accurate and the phenomenon of emulsification can not be found. Key words: tramal solid-phase extraction SKF 525A GC/MS 曲马多 tramal又名反胺苯环醇 为非阿片类中枢性镇痛药的一种 可用于中 [1] 度或严重的急慢性疼痛 但是 近几年来也常被吸毒人员作为毒品的代用品或消除毒 瘾的缓解剂而滥用 严重地干扰了正常的辑毒工作 为了更好地打击犯罪 维护社会治 安的稳定 我们应用固相萃取技术 结合GC/MS法对人体尿液中的曲马多进行了检验研 究 并取得了满意的结果 现报告如下 1实验部分 1.1仪器与试剂