芪蛭降糖胶囊对2型糖尿病大鼠肝组织中InsR，PI3K，GLUT2，p―JNK蛋白表达的影响.docVIP

芪蛭降糖胶囊对2型糖尿病大鼠肝组织中InsR，PI3K，GLUT2，p―JNK蛋白表达的影响 　　[摘要] 观察芪蛭降糖胶囊对2型糖尿病大鼠降糖的效果，并探讨其降糖的初步作用机制。该实验采用高糖高脂配合小剂量链脲佐菌素（STZ）制备成2型糖尿病大鼠模型，连续给药6周后，检测各组动物血糖、糖化血清蛋白（GSP）水平；免疫组化法检测胰腺组织中胰岛β细胞的数量；蛋白印迹法检测肝组织中胰岛素受体（InsR）、磷脂酰肌醇-3激酶（PI3K）、葡萄糖转运体2（GLUT2）及磷酸化的c-Jun氨基末端激酶（p-JNK）蛋白的表达。结果显示，芪蛭降糖胶囊能够降低糖尿病动物的血糖和血清GSP水平，提升血清中胰岛素含量和胰岛β细胞的数量，提高肝组织中InsR，PI3K，GLUT2蛋白表达，降低p-JNK蛋白的表达。总之，芪蛭降糖胶囊降糖的作用较为稳定，其降糖机制可能与增加胰岛β细胞的数量而提升血清中胰岛素的含量，提高肝组织中InsR，PI3K，GLUT2蛋白表达并降低p-JNK蛋白表达从而减少肝组织的胰岛素抵抗有关。 　　[关键词]芪蛭降糖胶囊； 2型糖尿病； 胰岛素信号通路 　　[Abstract] To observe the hypoglycemic effect of Qizhi Jiangtang capsule in rats with type 2 diabetes， and investigate the preliminary mechanism of its hypoglycemic effect， type 2 diabetes rat models were established by high glucose and high fat combined with small dose of streptozotocin （STZ）. After continuous administration for 6 weeks， blood glucose， and glycosylated serum protein （GSP） levels were detected in all of the animals； immunohistochemistry assay was used to detect the number of islet β cells； Western blot assay was used to detect the protein expression levels of insulin receptor （InsR）， phosphoinositide-3 kinases （PI3K）， glucose transporter-2 （GLUT2） and phosphorylated Jun N-terminal kinases （p-JNK）in hepatic tissues. The results showed that Qizhi Jiangtang capsule could reduce the blood sugar and GSP levels in serum in animals with type 2 diabetes mellitus， increase the level of insulin in serum and number of islet β cells， increase the protein expression levels of InsR， PI3K and GLUT2， and reduce the level of p-JNK protein expression. In conclusion， Qizhi Jiangtang capsule has relatively stable hypoglycemic effect， and the mechanism may be associated with increasing the number of islet β cells and level of insulin in serum， up-regulating the protein expression levels of InsR， PI3K and GLUT2， down-regulating the level of p-JNK protein expression in hepatic tissues， and reducing the level of insulin in hepatic tissues. 　　[Key words] Qizhi Jiangtang capsule； type 2 d