阳离子型疏水改性聚氧乙烯HMEO单组分双水相体系的相行为.pdfVIP

A005 阳离子型疏水改性聚氧乙烯(HM-EO) 单组分双水相体系的相行为 邓刚 姚善泾 * 林东强 （浙江大学化学与生物工程学系，杭州 310027） 摘 要 对阳离子型疏水改性聚氧乙烯(HM-EO)水溶液的相行为进行了研究。HM-EO在水溶液中 呈现两亲性，可以形成胶束，进而形成胶束簇集体。所形成胶束聚集体具有带电性，而且通过改 变溶液的pH值，盐浓度及相反电荷的表面活性剂SDS，均可改变胶束聚集体的带电状态。盐的种 类对HM-EO水溶液的相行为也有显著的影响，研究表明在增加双水相区域的效率上，阳离子的 作用大小次序是: K+＞Na+；而阴离子的次序则为: SO42-＞F-＞Cl-＞Br-＞I-。通过测定透光度和 电导率考察了HM-EO和阴离子表面活性剂SDS的相互作用，结果表明SDS能与HM-EO形成混合胶 束簇集体。 关键词 双水相 疏水改性聚氧乙烯 静电作用 胶束聚集体 PHASE BEHAVIOR OF A CATIONIC HYDROPHOBICALLY MODIFIED ETHYENE OXIDE POLYMER (HM-EO) IN AQUEOUS SOLUTION DENG Gang YAO Shanjing and LIN Dongqiang (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027) Abstract The phase behavior of a cationic hydrophobically modified ethylene oxide polymer(HM-EO) in water solution was investigated. The amphiphilic nature of HM-EO results in micellization or aggregation of micellars. The micellar aggregates can be charged and their netcharge can be changed by adjusting the pH and conductivity of the bulk solution and sodium dodecyl sulfate(SDS). The effect of different salts on the phase behavior of HM-EO aqueous solution was very significant. The interaction sequence of cations expressed as K+＞Na+, and the one of anions as SO42-＞F-＞Cl-＞Br-＞I-. The interaction between polymer and anion surfactant was studied by determination of transmittance and conductivity. The addition of SDS to the cationic HM-EO polymer causes the formation of mixed micellar aggregates of SDS and HM-EO. Keywords Aqueous two-phase system, Hydrophobically modified ethylene oxide polymer(HM-EO), Electrostatic interaction, Micellar aggregates 引 言 双水相分配技术由于其分配环境温和，易于操作和放大等优点，近二十年来，已被广泛 用于各种生物分子的分离纯化[1]。为了进一步改善双水相分配的选择性系数，提高分配效率， 对新型的成相聚合物的研究也不断深入，其中Malin和Thuresson等人[2, 3]提出的疏水改性聚氧 乙烯(HM-EO)是一种区别于传统双水相系统的新型成相剂，其主要特点在于其分子量较高 (约为 25000)、浊点温度低及在水溶液中能形成单组分双水相系统。由于HM-EO在水溶液中 能形成一种特殊的鸟巢状带电结构，相间的主要推动力来自于静电作用力，从而有望增加一 * 通讯联系人，E.mail: yaosj@che.zju.edu.c