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Improved high performance liquid chromatographic.pdf

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Improved high performance liquid chromatographic

Copyright ? 2008 John Wiley Sons, Ltd. Biomed. Chromatogr. 22: 637–644 (2008) DOI: 10.1002/bmc Simultaneous determination of 12 cytotoxic caged xanthones in gamboges 637ORIGINAL RESEARCHOR GINAL ESEARCH BIOMEDICAL CHROMATOGRAPHY Biomed. Chromatogr. 22: 637–644 (2008) Published nlin 6 February 2008 in Wiley InterScience () DOI: 10.1002/bmc.981 Improved high-performance liquid chromatographic method for simultaneous determination of 12 cytotoxic caged xanthones in gamboges, a potential anticancer resin from Garcinia hanburyi Song-Lin Li, Jing-Zheng Song, Quan-Bin Han, Chun-Feng Qiao and Hong-Xi Xu* Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, N. T., Hong Kong, People’s Republic of China Received 18 October 2007; revised 12 November 2007; accepted 18 November 2007 ABSTRACT: The potential anti-tumor activity of gamboges, a herbal medicine derived from Garcinia hanburyi, has increasingly gained the interest of scientist worldwide. The major components of gamboges are cytotoxic caged xanthones. In the present study, an improved HPLC method was developed to simultaneously quantify 12 caged xanthones, including three pairs of epimers and four pairs of trans–cis isomers, i.e. forbesione, isomorellic acid, morellic acid, R-30-hydroxygambogic acid, S-30-hydroxygambogic acid, isogambogenic acid, gambogenic acid, gambogellic acid, R-isogambogic acid, S-isogambogic acid, R-gambogic acid and S- gambogic acid. This method was validated to be sensitive, precise and accurate with limits of detection of 0.03–0.08 μg/mL, overall intra-day and inter-day variations less than 7.9% and overall recovery over 93.2%. The correlation coefficients (r 2) of the calibra- tion curves were higher than 0.995 for all analytes. The newly established method was successfully applied to reveal the difference in the chemical profiles and contents of these analytes in gamboges from different origins. It can be concluded that this method was not only an effecti