应用差异凝胶电泳及质谱技术筛选肺癌患者血浆差异蛋白.pdfVIP

上海交通大学学报(医学版) Vol. 30 No.4 Apr. 2010 Joumal of Shanghai Jiaotong University( Medical Science) .399 ? 文章编号: 1674-8 115(2010)04-0399-05 ·抢著· 应用差异凝胶电泳及质谱技术筛选肺癌患者血浆差异蛋白 沙慧芳E ， 孙强玲， 杨晓华E ， 储天睛z ， 谢银银3 鲁 静气 包国良， 玛久贤1 ， 龚乐罗 (上海交通大学胸科医院1.基础研究室， 2. 肺内科，上海 20∞30; 3. 医学院瑞金医院血液学研究所，上海 2∞025) 摘 要:曲曲曲建立健康者、肺部炎症患者和肺癌患者的血浆蛋白表达谱，寻找与肺癌早期发生相关的差异蛋白。方旅应用差 异凝胶电泳( DIGE) 和基质辅助激光解吸飞行时间质谱( MALDI-TOF-MS)对健康者、肺部炎症患者和 I 期肺癌患者血浆进行蛋 白质组学比较研究，寻找肺癌相关差异蛋白。稽..应用 DIGE 进行分离后，成功获得了蛋白质组分辨率高、重复性好的双向凝 胶电泳图谱;DeCyder 6. 5 软件分析获得差异大于 2 倍的蛋白质点共有 72 个 z质谱鉴定去冗余后确定了 12 种蛋白质，其中 7 种 与肿瘤相关，分别为酿基辅酶 A 合成酶家族成员 3、抗血小板膜糖蛋白 1 b 特异性抗体、-y-纤维蛋白原、4 型血清淀粉样蛋白 A 、 补体因子 H、钙结合微丝蛋白、载脂蛋白Io f告份应用 DIGE 及 MALDI-TOF-MS 分离并初步鉴定了 12 种蛋白质，筛选出 7 种在 肺癌患者血浆中离表达的肿瘤相关蛋白。 关键词:差异凝胶电泳;蛋白组学:血浆;肺癌 中图分类号: R734.2 文献标志码 :A Screening of differentially expressed serum proteins of lung cancer by DIGE and MALDI-TOF MS/MS SHA Hui-fang l , SUN Qiang-ling l , YANG Xiao-hu矿， CHU Tian-qing2 , XIE Yin-yin气 LU Jin矿， BAO Guo-liangl , FENG Jiu-xian l , GONG Le-luo l (1. ?asic Research Laboratory, 2. Department 01 Respiratory Medicine, Chest Hospital, Shanghai }iaotong University, Shanghai 2α1030， Chína; 3. lnstítute 01 Hematology, Ruíjín Hospítal, School 01 Medícíne, Shanghaí Jiaotong Universíty. Shanghai 200025. China) Abstract: Objective To establish plasma protein spectrum from healthy people, patients with pneumonia and patients with lung cancer, and seek differentially expressed proteins related to early development of lung cancer. Methods Differential gel electrophoresis (DIGE) and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI- TOF-MS) were employed to screen differentially expressed plasma proteins among healthy people, patients with pneumonia and patients with stage 1 lung cancer. Results Two dimensional gel electrophoresis patterns of plasma proteins with high-resolution and reproducibility were successfully obtained using DIGE. Seventy-two significantly differentially expressed protein spots were s