分光光度计法测定甲基红离解常数实验数据处理5页.doc

V、Data treatment Part 1：Measure the maximum absorption Table 1: Absorption of solution AWavelength/nmabsorption4200.0754400.1724600.414800.7625001.145101.2865201.3535301.3325401.2635600.9055800.2956000.046 Graph 1: Absorption of solution A Conclusion：from the Graph 1，we can get the wavelength for the maximum absorption of solution A is 520 nm. Table 2: Absorption of solution B Wavelength/nmabsorption4100.524200.5434300.5524400.5524500.544600.4984800.3545000.1785200.0745400.037 Graph 2: Absorption of solution B Conclusion：from the Graph 2，we can get the wavelength for the maximum absorption of solution A is 430-440 nm. Part 2：Test the Beer’s law Table 3: Absorption of HMR in different concentrationsNo.(concentration)Absorption (520nm )Absorption (430nm )0#-c(A)1.3530.1471#-0.75c(A)1.0640.1062#-0.5c(A)0.680.0643#-0.2c(A)0.3730.055Note：because it is always acidic through the process, we can ignore the absorotion of MR-. Graph 3：Absorption of HMR in different concentrations（520nm ) (the forth data is ignored due to its large derivation) Graph 4：Absorption of HMR in different concentrations（430nm ) Conclusion：(C0=1.851*10-5) from the Graph 3，the slope is 1.2510, so §HNM520=1.2510/(C0=1.851*10-5)=6.79*104，from the Graph 4，the slope is 0.166, so §HNM430=0.166/(C0=1.851*10-5)=8.97*103 . Table 4: Absorption of MR- of different concentrationsNo.(concentration)Absorption (520nm )Absorption (430nm )0#-c(B)0.0740.5521#-0.75c(B)0.04550.2882#-0.5c(B)0.04790.1343#-0.2c(B)0.03220.064Note：because it is alkaline through the process, we can ignore the absorotion of HMR. (the second data is ignored due to its large derivation) Graph 5：Absorption of MR- in different concentrations（520nm ) (the forth data is ignored due to its large derivation) Graph 6：Absorption of MR- in different concentrations（430nm ) Conclusion：(C0=1.851*10-5) from the Graph 5，the slope is