西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响_刘世红.pdf

植物生理学报 Plant Physiology Journal 2011, 47 (5): 505~511 505 西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响 刘世红*, 田耀华, 魏丽萍, 周会平 云南省热带作物科学研究所, 云南景洪666100 摘要: 采用低温恒温槽处理离体叶片后测定其电解质外渗率, 并结合Logistic方程得出低温半致死温度的方法, 评价农业部 景洪橡胶树种质资源圃中的30个橡胶树品种的抗寒性。结果表明, 品种‘RRIM712’、‘云研80-1983’、‘云研77-4’、 ‘PR107’、‘RRIM524’抗寒性强, 低温半致死温度在–4~0 ℃之间; 品种‘云研76-398’、‘RRIM523’抗寒性弱, 低温半致死温度 在4~7 ℃之间; 其他品种抗寒性居中。橡胶树在低温胁迫下, 超氧阴离子的产生速率与橡胶树的抗寒性无直接关系, 但不 同温度下的变化幅度越小, 抗寒性越高; SOD活性随温度降低迅速提高, CAT随温度降低表现出活性降低的趋势。 关键词: 橡胶树; 低温; 半致死温度; 抗氧化系统 Semi-Lethal Low Temperatures and Impact of Low Temperature on Antioxi- dant System of 30 Varieties of Rubber Trees in Xishuangbanna LIU Shi-Hong*, TIAN Yao-Hua, WEI Li-Ping, ZHOU Hui-Ping Yunnan Tropical Crops Research Institute, Jinghong, Yunnan 666100, China Abstract: To evaluate the cold tolerance of 30 varieties of rubber trees planted in Jinghong rubber tree germ- plasm resources garden of ministry of agriculture, its leaves were treated by low-temperature thermostat bath, then its electrolyte leakage were measured, the semi-lethal low-temperature were obtained using Logistic equa- tion. The results show that the cold resistances of variety ‘RRIM712’, ‘YUNYAN 80-1983’, ‘YUNYAN 77-4’, ‘PR107’ and ‘RRIM524’ are strong, their semi-lethal low temperatures are between –4 ℃ to 0 ℃; those of vari- ety ‘YUNYAN 76-398’ and ‘RRIM 523’ are weak, their semi-lethal low temperatures are between 4 ℃ to 7 ℃; other varieties’ cold resistance are normal. Under chilling stress, the production rate of superoxide anion are not directly related to cold tolerance, but the smaller the change between different temperature, the higher the cold resistance. SOD activity increased rapidly with decreasing temperature, but CAT activity decreased in the ex- periment. Key words: rubber tree; low-temperature; semi-lethal temperature; antioxidant system 天然橡胶是现代工业不可或缺的四大原料之 陈国权1992; Lyons 1973)。因此, 通过测定植物相 一, 在交通、国防工业上更具有重大的战略意义。 对电导