胰岛素样生长因子结合蛋白3对非小细胞肺癌的作用.docVIP

KKME---专业医学搜索引擎/ 胰岛素样生长因子结合蛋白-3对非小细胞肺癌的作用 　　作者：皮佳俐 徐平 宋卫东 刘媛媛 朱棠 作者单位：北京大学深圳医院呼吸内科，广东 深圳 518036 　　【摘要】 目的 探讨胰岛素样生长因子结合蛋白-3(IGFBP-3)诱导非小细胞肺癌细胞(NSCLC)凋亡、抑制增殖的作用。方法 分别应用CCK-8法、流式细胞仪检测IGFBP-3对NCI-H520、A549细胞株体外增殖、细胞周期及调亡的作用，用倒置显微镜观察细胞形态学变化。结果 CCK-8实验表明：浓度0.05,0.1，0.2，0.4,0.8 Μg/ml 的IGFBP-3对NCI-H520、A549增殖有明显的抑制作用，且表现为剂量依赖性;流式细胞学显示：两株细胞在IGFBP-3作用后均出现凋亡峰，且S期的细胞减少，以A549更为明显，倒置显微镜下可见胞质浓缩、体积缩小，细胞碎裂。结论 IGFBP-3可抑制NSCLC增殖，诱导凋亡。 　　【关键词】 非小细胞肺癌;胰岛素样生长因子结合蛋白-3;凋亡;增殖 　　基金项目：广东省医学科研基金(编号：A2006606) 　　Effect of insulin like growth factor binding protein 3 on non-small cell lung cancer 　　PI Jia-Li, XU Ping, SONG Wei-Dong, et al. 　　Department of Respiration of Beijing University Shenzhen Hospital, Shenzhen 518034, Guangdong, China 　　【Abstract】 Objective To investigate the effect of insulin-like growth factor binding protein (IGFBP)-3 on inducing apoptosis and inhibiting proliferation of human non-small cell lung cancer (NSCLC).Methods The inhibitory effect of IGFBP-3 on proliferation of NCI-H520 and A549 cell lines in vitro was detected by CCK-8 assay.The cell cycle arrest and apoptosis were observed by flow cytometry. The morphological change was observed by inverted microscope. Results CCK-8 assay showed that IGFBP-3 at the concentration of 0.05, 0.10, 0.20, 0.40, 0.80 Μg/ml significantly inhibited proliferation of A549 and NCI-H520 cells in a dose dependent.Flow cytometry analysis showed that IGFBP-3 induced S phage arrest and high peak of cell apoptosis of A549 and NCI-H520 cells, especially in A549. Under inverted microscope, some A549 and NCI-H520 cells with condensed cytoplasm, loss of volume and broken cell were seen. Conclusions IGFBP-3 could inhibit proliferation, and induce apoptosis of NSCLC cells. 　　【Key words】 Non-small cell lung cancer;Insulin-like growth factor binding protein 3; Apoptosis; Proliferation 　　肺癌的发病率呈逐年上升趋势。尽管新的化疗药物不断问世，但经手术、放疗和化疗在内的综合治疗后，其效果仍不理想，总的5年生存率美国在15%以下，我国则更低。肿瘤的发生是细胞增殖与凋亡失控的结果，也是细胞周期失控的结果。生长激素-胰岛素样生长因子轴在调控细胞生长及凋亡