NBD多肽治疗实验性溃疡性结肠炎的实验研究的论文.docVIP

　　NBD多肽治疗实验性溃疡性结肠炎的实验研究的论文 作者：杨剑,崔淑兰,龙友明,陈垦,王晖,王念林,谢文瑞,刘君君 【摘要】 　　目的 观察核因子κb必需分子(nfκb essential modulator，nemo)结合的小分子多肽(micromolecule polypeptide bining obinding domain，nbd多肽)对实验性大鼠溃疡性结肠炎的治疗作用。方法 60只sd大鼠随机分成治疗组、对照组各30只，采用三硝基苯磺酸(tnbs)灌肠法制作溃疡性结肠炎大鼠模型，治疗组予腹腔注射1.3 mg/100 g体质量的nbd多肽，对照组予等量生理盐水，评估炎症活动指数（iai），给药后7 d处死相应组的动物取结肠，肉眼观察结肠黏膜病变且按损伤情况积分，行病理切片、苏木素伊红(hematoxylin eosin，he)染色，光镜下评估组织损伤；取病变结肠组织切片行免疫组化检测核因子κb(nfκb)表达，检测髓过氧化物酶(mpo)、丙二醛(mda)。结果 治疗组大体形态损伤、组织学损伤评分、iai评分分别为2.41±0.43、4.80±0.82、6.02±0.59，明显低于对照组(3.50±0.51、6.90±1.13、3.05±0.51)(plt;0.05)；治疗组mda、mpo及nfκb表达均低于对照组(plt;0.05)。.结论 nbd多肽对于溃疡性结肠炎大鼠模型有较好的治疗作用，其作用与其抑制nfκb表达有关。 【关键词】 nbd多肽 溃疡性结肠炎 动物模型 nf κb 免疫组化 　　abstract:objective to observe the timeactivitycurve of the therapeutic effect of micromolecule polypeptide bining obinding domain on experimental ulcerative colitis rat. methods sixty sd rats ly divided into treated and control group. ulcerative colitis rat model icromolecule polypeptide bining obinding domain inistered by intraperitoneal in treated group， and ejust doses liquor natrii chloridi isotonicus inistered likely in control group. iai score odel ade for the observation of the colonic pathologic changes by naked eye and for the estimation of the damage scores of colon mucosa. finally，the colon tissue ake pathological section and the damage score of tissue icroscope. and then damaged colon tissues pathological section test expression of nfκb by immunizing histochemistry. then the value of mda、mpo in supernatant of sampless damaged colon tissues divide evenly an oar damage scores、damage scores of tissue、iai score are 2.41±0.43、4.8±0.82、6.2±0.59， and they all are lopo、mda、nfκb are loicromolecule polypeptide bining obinding domain has protective effect against rat ulcerative colitis，and its mechanism relat odulatorbinding domain (nbd) peptide; ulcerative colitis; animal model; nfκb; immunohistochemistry 　　溃疡性结肠炎(uc)即慢性非特异性溃疡性结肠炎，是一种病因尚未完全明确的直肠和结肠的慢性炎症性疾病，病程迁延，有癌变趋势，近年来患病率呈上升趋势。目前研究发现核转