紫杉醇脂质体节奏性化疗对胃癌乏氧影响的实验研究的论文.docVIP

　　紫杉醇脂质体节奏性化疗对胃癌乏氧影响的实验研究的论文 【摘要】 ［目的］ 利用乏氧显像监测紫杉醇脂质体节奏性化疗过程中胃癌的乏氧变化。［方法］36只荷人胃癌hs-746t细胞的雄性裸鼠（4~6周）随机分成两组，治疗组按5mg/kg的剂量每3天一次行腹腔内注射紫杉醇脂质体，对照组注射同体积的生理盐水。治疗后第2d和14d利用spect全身静态扫描显示注射99mtc-hl91后5，10，15，30，60，120和180min的显像。在显像上标记感兴趣区为靶点，计算各组靶/非靶比值。然后处死裸鼠、切下肿瘤行称重和放射性计数。通过免疫组织化学染色检测cd34表达，计算肿瘤微血管密度（mvd）。 ［结果］ 治疗后第2d，紫杉醇脂质体治疗组t/nt低于对照组（7.82±0.28 vs. 9.56±0.31，plt;0.05），离体肿瘤放射性滞留降低（0.71±0.03 vs. 0.97±0.03，plt;0.05），mvd降低（42.89±2.63 vs. 52.78±3.09，plt;0.05）；治疗后第14d，治疗组t/nt高于对照组（11.54±0.42 vs. 9.96±0.28，plt;0.05），离体肿瘤放射性滞留升高（1.42±0.05 vs. 11.54±0.42，plt;0.05），mvd明显降低（27.89±2.51 vs. 66.11±2.83，plt;0.001）。 ［结论］ 治疗初期紫杉醇脂质体降低胃癌乏氧，后期乏氧增加，乏氧监测对胃癌的治疗有一定的指导意义。 【关键词】 胃癌细胞 experimental study of effects of metronomic chemotherapy es on gastric cancer hypoxia zheng yong-bin, tong shi-lun, he jie, et al. (renmin hospital of onitor the hypoxic change of gastric cancer during metronomic chemotherapy es by the hypoxia imaging. ［methods］ thirty-six male nude mice bearing human gastric cancer hs-746t cells ly divided into tent group received paclitaxel-liposomes at a dose of 5mg/kg intraperitoneally and control group received the same volume of saline in every three days. on 2 and 14 days, all the mice underaging inutes after injection of 99mtc-hl91. region of interest, roi agines and target-to-non targeted (t/nt) ice ors oved, easured. cd34 expressions munohistochemical staining and microvascular densities (mvd) of the tumors es treatment, t/nt ratio of treatment group vds of isolated tumor tissues in the treatment group ent, t/nt ratio and the radioactive retention of treatment group vd of isolated tumor tissues in the treatment group ent, paclitaxel-liposomes decrease the hypoxia of gastric cancer and in the late stage of treatment or hypoxia. therefore, detection tumor hypoxia may be important in guiding the treatment for gastric cancer. key tc-hl91; paclitaxel-liposomes; tumor hypoxia; nude mice 多数实体瘤中存在不同程度的乏氧细胞，乏氧是肿瘤异质性的重要特征。.实验及临床数据显示乏氧不仅是导致放疗失败和化疗耐受的重要原因，而且使肿瘤具有侵袭性，易发生治疗后复发和远处转移