-opioid receptor stimulation on angiotensin -induced inhibition of cardiac hypertrophy.docVIP

 PAGE \* MERGEFORMAT 16 -opioid receptor stimulation on angiotensin -induced inhibition of cardiac hypertrophy Of: Zhang, Wang and new Guo-Qiang Wu, Jie [Abstract] Objective To observe the -opioid receptor stimulation by angiotensin II induced cardiac hypertrophy on the role of opioid receptor agonists on cardiac hypertrophy induced by angiotensin II inhibition. Methods in cultured neonatal rat cardiomyocytes in vitro cells as a model, angiotensin II1 M induced cardiac hypertrophy, observed U50488H1 M of its role, and, and losartan (los1 M to do comparison group observed the activation of -opioid receptor on cardiac hypertrophy. with the Lowry assay protein content of myocardial cells, isolated by digestion method and computer image analysis system for detection of myocardial cell volume, using [3H]-leueine incorporation of myocardial cells in protein synthesis. Results AngII1 M total protein content of myocardial cells and protein expression and cell volume increased significantly, U50488H1 M to reduce the AngII induced myocardial cell protein content, protein expression, and the increase in volume, thereby inhibiting cardiac hypertrophy, and the degree of inhibition similar with los1 M. Conclusions -opioid receptor agonist can inhibit the AngII induced U50488H myocardial cell hypertrophy. [Keywords:] cardiac hypertrophy Angiotensin II U50488H opioid receptor Abstract: Objective To demonstrate the inhibitory effect of -Opioid on AngII-induced hypertrophy in cultured myocardial cells of neonatal rats. Methods Based on the model of cardiac bistiocyte cells in vitro culture, the experiment applies the angiotensin II1 M to induce the myocardium to be hypertrophy. Compared with the group of (los) 1 M, the experiment observes the effects of the activation of -Opioid on myocardial hypertrophy. The protein content was measured by Lowry s method. The protein synthetic rate was obtained through measuring the incorporation of [3H]-leueine into myocyte prot