1 transforming growth factor-induced A549 cells to mesenchymal cell transformation in the role of.docVIP

 PAGE \* MERGEFORMAT 12 1 transforming growth factor-induced A549 cells to mesenchymal cell transformation in the role of Of: Zhengjin Xu, Chang Hsiao-yen, end Li-Rong, Chen Wei, Shu-hong tube [Abstract] Objective: To observe the recombinant human transforming growth factor 1 (rhTGF 1) on human lung epithelial cell line (A549) transformation. METHODS: Cultured A549 with different concentrations of rhTGF 1 after the intervention of cell morphology was observed by inverted microscope changes in school by MTT assay rhTGF 1 on the proliferation of .48 h A549 collected protein Western blot and indirect immunofluorescence using chemical methods to detect mesenchymal phenotype Fibronectin EDA (Fn EDA expression. Results: rhTGF 1 induction of A549 to Transformation of mesenchymal cells in vitro had no effect on A549 proliferation, increased mesenchymal cell phenotype Fn EDA expression. Conclusion: rhTGF 1 could induce epithelial cells A549 happen - mesenchymal transition (ephethlial mesenchymal transition, EMT), support lung epithelial cells EMT point of view. [Keywords:] transforming growth factor 1, human lung epithelial cell line (A549), epithelial - mesenchymal transition [Abstract] Objective: This experiment was designed to investigate the differentiatied role of the recombinal human tranfoming growth factor beta 1 (rhTGF 1) on one strain of human alveolar epithelial cells.Methods: Cultured A549 cells were treated at different concentration, the morphology change observed under phase contrast microscopy; the effect of proliferation of A549 were detected by MTT. The Fibronectin EDA expession were analysised of cell lysates by Western blotting after 48 h and inderict Eneyme inmmuohistorchemitery (IEI). Results: The data showed that rhTGF 1 induced A549 cells morphological alteration from alveolar epithelial to mesenchymal, ineffected with A549 cells proliferation, up regulation of the mesenchymal phenotype Fibronectin EDA marker. Conclusion: Our study sh