1,25 - dihydroxy vitamin D3 on streptozotocin-induced type 1 diabetes in mice and mechanism of preventive effect of.docVIP

 PAGE \* MERGEFORMAT 21 1,25 - dihydroxy vitamin D3 on streptozotocin-induced type 1 diabetes in mice and mechanism of preventive effect of 【Abstract】 Objective 1,25 - dihydroxyvitamin D3 [1,25 - (OH) 2D3] on the continuous low-dose streptozotocin multiple (the multiple low dose streptozotocin, MLDS)-induced prevention of type 1 diabetes in mice effect and its mechanism. Preparation method MLDS intraperitoneal injection of type 1 diabetes in mice models to blood glucose levels consistently greater than 16.7mmol / L into a mold of the standard. Experiment is divided into three groups, normal control group, diabetes group, prevention groups. Using a portable blood glucose meters to monitor blood glucose levels; radioimmunoassay of serum insulin levels in each group; Immunohistochemistry was used to detect islet cells of each group of apoptosis-related proteins Bax and Bcl-xL expression. Results MLDS-induced model of type 1 diabetes was basically completed in the fourth week. MLDS of mice blood sugar, serum insulin levels decreased, Bax increase, Bcl-xL down. Prevention of decline in blood glucose levels, serum insulin increased, Bax down, Bcl-xL increases, decreased incidence of diabetes, and diabetes group were significantly different (P lt;0.05). Conclusions 1,25 - (OH) 2D3 could be effective in preventing MLDS induced the occurrence of type 1 diabetes, its effects may be related to 1,25 - (OH) 2D3 regulate Bax and Bcl-xL protein expression to be balanced. Keywords: 1,25 - dihydroxy vitamin D3; streptozotocin; 1 diabetes mellitus; Bax; Bcl-xL Abstract: Objective To investigate the prevention and the mechanism of 1,25-dihydroxyvitamin D3 to type 1 diabetes by streptozotocin-induced in KM mice.Methods Experimental type 1 diabetes mice model with the blood glucose level which was consistently higher than 16.7mmol / L were produced by intraperitoneal injection of MLDS.There are three groups in this studay, which were normal control group, d