3 - Daidzein Sulfonate on endothelial cells induced by oxidized low density lipoprotein protective effect of injury.docVIP

 PAGE \* MERGEFORMAT 12 3 - Daidzein Sulfonate on endothelial cells induced by oxidized low density lipoprotein protective effect of injury Of: have Jing, Huang Yuping, Huang Yushan, Wang Shuren [Abstract] Objective To study the 3’-daidzein sulfonate sodium (DSS) induced by oxidized low density lipoprotein on human umbilical vein endothelial cell injury and its mechanism. Methods Cultured human umbilical vein endothelial cells, the cells were divided into control group, oxidative damage in control group (ox-LDL), oxidative damage by adding vitamin E control group (VE + ox-LDL), oxidative damage by adding DSS low, medium and high concentration group (DSS-L, DSS-M and DSS-H group). the role of ox-LDL at different concentrations of pre-accession DSS VE and 24 h pretreatment of endothelial cells cultured for 24h, MTT cell viability assay, colorimetric detection of malondialdehyde (MDA) and nitric oxide (NO ) content, lactate dehydrogenase test (LDH), superoxide dismutase (SOD), nitric oxide synthase (NOS) and glutathione peroxidase (GSH-Px) activity. The results pre-accession VE, the high concentration of DSS can enhance the damage of endothelial cell viability, can significantly reduce the injury of endothelial cells MDA and NO release, can significantly enhance the damage cells, LDH, NOS, SOD and GSH-Px activity, but low concentrations DSS has little effect on these indicators. Conclusions DSS reduce ox-LDL on vascular endothelial cell oxidative damage, play a role in antioxidant protection. [Keywords:] 3’-daidzein sulfonate; oxidized low density lipoprotein; endothelial cells; protective effect Abstract: ObjectiveTo investigate the protective effect and mechanisms of 3’-daidzein sulfonate sodium on vascular endothelial cell injury induced by oxidized low-density lipoprotein. MethodsHuman umbilical vein endothelial cells (HUVECs) were cultured in vitro and divided into 5 groups: control, ox -LDL, VE + ox-LDL, DSS-L, DSS-M and DSS-H groups. Endothelial