4 integrin-binding protein regulation of extracellular matrix fibroblasts Experimental study build.docVIP

 PAGE \* MERGEFORMAT 18 4 integrin-binding protein regulation of extracellular matrix fibroblasts Experimental study build Of: Tanjiang Lin, Peng Xu, Yuan Shunzong, horsemen, and Earnestly Hu Xiaohong, Luogao Xing, Wu Jun [Abstract] Objective To investigate the 4 integrin binding protein (ITGB4BP) on fibroblast cell regulation of extracellular matrix formation. Methods were constructed, packaged in mammalian cells can promote and inhibit the expression of adenovirus ITGB4BP pAdEasy ITGB4BP EGFP expression vector and Lentiviral vector Lentivirus FIV H1/U6 copGFP ITGB4BP RNAi, identified by screening valid, were infected with the human normal skin fibroblasts and hypertrophic scar (HTS) fibroblasts, cell culture supernatant by ELISA detection of MMPs ( MMP 9), transforming growth factor 1 (TGF 1) and type collagen protein (collagen ) expression. the result of normal skin fibroblasts ITGB4BP overexpression, caused MMP 9, TGF 1 and decreased expression of collagen ; contrary HTS fibroblasts ITGB4BP expression inhibition, MMP 9, TGF 1 and increased expression of collagen . Conclusion ITGB7BP of MMP 9, TGF 1 and collagen expression of important regulatory functions. ITGB4BP may have anti-fibrosis suggest an important function, as Next in-depth study of the formation mechanism of HTS provides an important basis. [Keywords:] 4 integrin binding protein; adenovirus; slow virus; fibroblasts Abstract: Objective To identify the effect of ITGB4BP (integrin beta 4 binding protein) on the production of extracellular matrix in fibroblasts.Methods The recombinant adenovirus pAdEasy ITGB4BP EGFP and Lentivirus FIV H1/U6 copGFP ITGB4BP RNAi were constructed, packaged and amplified in packaging cells , respectively.The primary cultured fibroblast from human normal skin was infected by the recombinant adenovirus pAdEasy ITGB4BP EGFP, while the fibroblast isolated from human hypertrophic scar was infected by the Lentivirus FIV H1/U6 copGFP ITGB4BP RNAi.The expression of