Acid amyloid Lithium inhibition of protein secretion and the protective effects of neuronal.docVIP

 PAGE \* MERGEFORMAT 14 Acid amyloid Lithium inhibition of protein secretion and the protective effects of neuronal Of: Hou Jun Lu Zhang Xiaoyu Luo Huanmin on behalf of Road [Abstract] Objective To study the acid lithium on human amyloid precursor protein in early old hormone 1 (M146L cells secrete -amyloid protein (A1 42 and its effects on cultured mouse cortical neurons of newborn rats. Methods AD in vitro gene transfection may be secreted A1 42 of the M146L cells in the culture medium by adding low, medium and high dose (3.1,5,8 mmol / L lithium tungsten or positive control, cell activity and by M146L medium A1 42 to the determination of acid lithium cells A1 42 M146L effect. serum-free culture in vitro neonatal mouse cortical neurons by NSE immunocytochemical staining for neuronal identification, observation of neuronal neurite growth status, analysis of acid on cerebral cortex neurons of lithium neuroprotection. Results low, medium and high doses of lithium tungstate had no cytotoxicity on the M146L, low, medium and high doses of lithium inhibit the M146L W cells A1 42, primary cultures of neonatal mouse cortical neurons by NSE immunocytochemistry the cells were stained brown majority, indicating that the cultured cells into neurons basically, In addition to low concentrations ( 0.008 mmol / L, the final concentrations of 0.2,0.04 mmol / L lithium tungsten could significantly increase the number and length of processes, may promote the survival of neurons. Conclusion lithium can inhibit the amount of acid secreted A1 42 M146L cells , cortical neurons of newborn mice have neuroprotective effects. [Key Words] lithium tungsten, Alzheimer’s disease, M146L cells, amyloid, neuroprotection [Abstract] Objective To explore the effects of lithium tungstate (LT) on the secretion of amyloid beta protein 1 42 (A1 42of M146L and the neuropretection on neurons from cerebral cortex of newborn mouse in vitro. Methods M146L cells were culture by low, mid