Acrostichum mangrove endophytic fungus Penicillium sp. 0935030 active ingredient in antibacterial research.docVIP

 PAGE \* MERGEFORMAT 16 Acrostichum mangrove endophytic fungus Penicillium sp. 0935030 active ingredient in antibacterial research Author: Cui Lin Haipeng Du Haibin Miao Cheng Wen-Li Mei Hong Kui Dai Yoshitomi [Abstract] for the study of mangrove plants Acrostichum (Acrostichum aureurm) endophytic fungi Penicillium sp.0935030 leavening ingredient in antimicrobial activity, using active tracking methods, and use a variety of chromatography separation and purification of the compounds, through the physical and chemical properties and spectral data identified seven compounds were as follows: Central (proline  threonine) (1), cyclo-(Proline  tyrosine) (2), 1  furyl ethylene  1,2  alcohol (3), (3β , 4β , 22β )  12  ene-3-hydroxy  3,22,23   oleanane (4), mannitol (5), uridine (6) and liquorice hormone (7 ). These compounds were first isolated from the fungus. Agar diffusion method using filter paper Determination of the antibacterial activity of these compounds, including compounds 1,2 and 7 have obvious anti-Staphylococcus aureus and methicillin-resistant Staphylococcus aureus activity. [Keywords:] halogen fern; endophytic fungi; Penicillium genera; cyclic peptide; antibacterial activity ABSTRACT To study the antibacterial constituents from the fermentation broth of endophytic fungus Penicillium sp. 0935030 from mangrove plant Acrostichum aureurm. The separation procedure was guided by antibacterial bioassay. Seven compounds were isolated and purified by column chromatography, and their structures were identified as cyclo ( Pro  Thr) (1), cyclo (Pro  Tyr) (2), 1  (2  Furanyl)  1,2  ethanediol (3), (3β , 4β , 22β )  olean  12  ene  3 , 22,23  triol (4), mannitol (5), uridine (6), and liquiritigenin (7) by physicochemical and spectral data. All compounds were isolated from this fungus for the first time. Antibacterial activities of all the compounds were assayed by paper disk diffusion method, and compounds 1, 2, and 7 sho