Agkistrodon halys venom-induced apoptosis of synovial cells in patients with rheumatoid arthritis experimental study.docVIP

 PAGE \* MERGEFORMAT 5 Agkistrodon halys venom-induced apoptosis of synovial cells in patients with rheumatoid arthritis experimental study Author: Xu Qing-Hua Lu Hu Jue Yang Xuyan China Eastern Airlines [Keywords:] Agkistrodon halys venom rheumatoid arthritis synovial cell apoptosis in Rheumatoid arthritis (RA) is a kind of synovial hyperplasia, lymphoid infiltration of synovial layer of macrophages, accompanied by a small amount of apoptosis in autoimmune diseases characterized. Past studies have shown that anti-apoptotic protein Bcl-2 in RA patients than normal in patients with osteoarthritis significantly up-regulated, thus inhibiting apoptosis of synovial fibroblasts and lead to abnormal proliferation of synovial membrane in patients with RA [1,2]. Agkistrodon halys Pallas (Agkistrodom halys Pallas venom, ASV) of the main active ingredient snake venom proteins induce apoptosis and anti-tumor effects in clinical practice has been used to treat rheumatoid arthritis and other chronic diseases caused by inflammation and pain [3, 4]. However, ASV on the mechanism of the treatment of patients with RA remain unclear. Authors from February to July 2007 on the ASV detection of RA synovial fluid inhibition of fibroblast proliferation and induce apoptosis. A clinical data 1.1 General Information Select Second Affiliated Hospital of Zhejiang University Orthopedics line of the RA patients with knee replacement surgery in 6 cases, including 3 males and 3 females; aged 34 to 62 years with an average (50 ± 10) years of age; course of 6 to 30 years, the average (14 ± 10) years. All patients are in compliance with 1987 American College of Rheumatology (ACR) revised diagnostic criteria for RA. 1.2 Main Reagents DMEM medium, Ⅱ -type collagenase, FBS, the United States Gibco Company; ASV from Zhejiang Dragon Figure Snake Industry Group Co., Ltd. to provide, -20 ℃ to save; Annexin V-FITC Apoptosis Detection Kit, the U.S. BD company; mouse anti-human bcl-2-FITC-mAb, goa