Akebia trifoliata fingerprint.docVIP

 PAGE \* MERGEFORMAT 10 Akebia trifoliata fingerprint [Keywords:] fingerprinting;,, Akebia trifoliata;,, Chuanmutong;,, high-performance liquid chromatography Abstract: Objective RP-HPLC (RPHPLC) to establish fingerprints of Akebia trifoliata. Method Hypersil ODS column, H2O and CH4 gradient elution, flow rate: 1ml/min, column temperature: 20 ℃ , detection wavelength: 215 nm. Resulted in the establishment of the HPLC fingerprint of a total model, and apply this common pattern of the five-leaf Akebia Akebia and Sichuan had similarity comparison. Conclusion herbs Akebia trifoliata were well in the separation of the components can be used as medicinal herbs Akebia trifoliata proprietary fingerprint. Keywords: fingerprinting; Akebia trifoliata; Chuan Mu Tong; high performance liquid chromatography Study on HPLC Fingerprint Chromatography of Akebia trifoliata (Thunb.) Koidz Abstract: ObjectiveTo apply RPHPLC to built the fingerprint of Akebia trifoliata (Thunb) Koidz. MethodsHypersil ODS column was used, with mixtures of water and methanol as mobile phase in a gradient mode. The flow rate was 1 ml / min. The temperature of column was 20 ℃ . The wavelength of detection was 215 nm. ResultsAfter the nutual mode of HPLC fingerprint was set up, comparison of similar degree to the crude drugs of Akebia quinata (Thunb.) Decne. and Clematis montana Buch.Ham. had been carried on. ConclusionAccording to the selected chromatographic conditions, a good fingerprint of Akebia trifoliata (Thunb) Koidz has been described. The method is simple, accurate with good reproducibility. It may be practical value for the quality control of sample for Akebia trifoliata (Thunb) Koidz. Keywords:: Fingerprint chromatography; Akebia trifoliata (Thunb) Koidz.; Chuan Mutong; HPLC Lardizabalaceae plant Akebia trifoliata Akebia trifoliata (Thunb) Koidz.’s Dry cane taste slightly bitter, cold, heat, diuresis, Huoxuetongmai, there is pain, sterilization, anti-inflammatory, diuretic’s effect,