Alisma Extract free radical scavenging capacity of.docVIP

 PAGE \* MERGEFORMAT 13 Alisma Extract free radical scavenging capacity of [Abstract] Objective To study extracts of different parts of Alisma free radical scavenging activity. Methods using organic solvent extraction and macroporous resin elution method will be divided into the total extract of Alisma petroleum ether, ethyl acetate part of the n-butanol fraction, ethanol portion of ethanol and water, to mention some parts of elution. Study of the hydroxyl radical, respectively (* OH), superoxide anion radical (O2-* ), DPPH free radical (DPPH * ) and the effect of H2O2 removal. Results Alismataceae n-butanol part of the clearance of O2-* and H2O2 resistant and some ethyl acetate removed DPPH * stronger, while the water extracts of Alisma orientalis part of stronger ability remove OH radicals, but was better than vitamin C. Conclusion Alismataceae n-butanol fraction, ethyl acetate and water, put some part of the free radical scavenging ability. [Keywords:] Alisma superoxide anion hydroxyl radicals DPPH Abstract: ObjectiveTo investigate free radical scavenging activity in vitro of different polarity fractions of extracts from Alisma orientalis. MethodsSix different fractions were obtained from the extract of A. orientalis by using different polarities of solvents, such as petroleum ether, ethyl acetate, n-butanol , ethanol, water, and macroporous resin. According to scavenging activities for hydroxyl radical (* OH), superoxidized radical (O2-* ), 1,1-diphenyl-2-picrylhydrazyl (DPPH * ) radical and H2O2, free radical scavenging activities of different polarity fractions were compared.Resultsn-butanol fraction of A. Orientalis had better scavenging ability than others for O2-* and H2O2, ethyl acetate fraction of A. Orientalis had better scavenging ability than others for DPPH * , water fraction of A. Orientalis had better scavenging ability than others for * OH, including Vitamin C. ConclusionThe fractions of ethylacetate, n-butanol and water have strong f