Allogeneic bone marrow mesenchymal stem cell transplantation for treatment of bone defects in animal experiments.docVIP

 PAGE \* MERGEFORMAT 14 Allogeneic bone marrow mesenchymal stem cell transplantation for treatment of bone defects in animal experiments Authors: Fu, Liang Haixiang, He loves Shan, Lei Lei, Lin Hong, Sheng Pu Yi, Yang Zibo, Liao Wei-ming 【Abstract】 【Objective】 examine the use of mixed allogeneic bone marrow mesenchymal stem cell transplantation for treatment of bone defects of the feasibility and preliminary results of animal experiments. 【Methods】 15 New Zealand white rabbits with bilateral radial bone defects caused by 1 cm model, randomly select one side of the same animal as the experimental side, auto-matching on the other side of the control side. The surface-demineralized allogeneic bone and from receptors in vitro proliferation of bone marrow mesenchymal stem cells implanted in experimental side mixed bone defect in the control side is only produced by the same allogeneic bone implants. After 12 weeks, X-ray examination, bio-mechanical examination and histological examination, the results were compared. 【Results】 animals 12 weeks after the experimental side X-ray film optical density measurements, the failure load when the torque and twist angle measurement results were compared with the control side; histological score in the experimental side the amount of score compared with the control callus side of the bone marrow to connect the degree of development maturity and that there is no significant difference. 【Conclusion】 bone marrow mesenchymal stem cells can promote bone allograft after transplantation of bone-forming role in the increase in bone volume without affecting the growth of bone tissue. Keywords: allogeneic bone; bone marrow mesenchymal stem cells; transplantation; bone defect Abstract: 【Objective】 To study the feasibility and result of transplanting bone allograft with bone marrow stem cell in treatment of bone defect in animal experiment. 【Methods】 Fifteen New Zealand white rabbitswere chosen for the 1 cm bone