Alcohol bone bank and its clinical application.docVIP

 PAGE \* MERGEFORMAT 7 Alcohol bone bank and its clinical application [Keywords:] alcohol clinical application of bone bank The use of bone bank bone allografts are many ways to save our hospital with accident injuries result in bone fractures in more and more cases of the actual situation of alcohol in animal experiments based on the bone bank, since November 2003 on the establishment of fetal alcohol bone bank, use the library repair of bone defects in 32 cases, effective and reliable, into the bone good effect. 1 Clinical data 1.1 General information on this group a total of 32 patients, 18 males and 14 females, aged from 12 to 63 years, mean 35 years. Fresh fractures, bone defects in 17 cases, 10 cases of delayed bone nonunion, bone defect of benign bone tumor resection in 5 cases. 1.2 Major sources for the bone health of more than 6 months abortion fetus or stillbirth, sampling criteria: (1 parents are healthy, tuberculosis, hepatitis, AIDS and other infectious diseases history. (2 dead babies instead of the normal induction of labor teratogenic, poisoning or stillbirth. (3 bones to be taken within the 6 h. 1.3 Preparation of bone bank in a clean condition, remove the long bone of fetal limbs, complete removal of the periosteum and muscular structure attached to plate cartilage, long bone longitudinal cut open to remove the marrow contents, first with 0.6 mol / L hydrochloric acid solution immersion 1 h, then washed in 70% alcohol, placed in sterile wide-mouth bottle, with 95% ethanol stored for 2 weeks and then transported to 70% alcohol, deposited in the 0 ~ 4 refrigerator. marked before storage Taigu months of age, take bone dates. once every 15 days to replace alcohol. 1.4 1 week before use to use alcohol should do bone bacteria culture 1 library, remove the fetus on the operating table bone sterile, saline soaked 15 min, to cut into a “match stick” like a small skeleton, closely filling the bone defect area or fracture fragments