Alprostadil on focal cerebral ischemia in rat brain tissue of p53.docVIP

 PAGE \* MERGEFORMAT 5 Alprostadil on focal cerebral ischemia in rat brain tissue of p53 Author: Cui-fang Tian cloth bag Cuifen package first Min Lian-qiu [Abstract] Objective: From the brain tissue changes in the expression of p53 in terms of alprostadil on the prevention and treatment of focal cerebral ischemia in rats mechanisms and dose-effect relationship. Methods: The middle cerebral artery occlusion method to establish animal models of focal cerebral ischemia, the effects of low, middle and high doses of alprostadil on focal cerebral ischemia in rat cerebral cortex p53 expression. Results: The rats in each group alprostadil p53 expression in brain tissue mean gray were 115.75 ± 11.38,140.13 ± 11.38,156.21 ± 10.05, higher than the ischemic control group, 97.91 ± 10.01. Conclusion: The prevention and treatment of alprostadil in rats after focal cerebral ischemia injury in brain tissue may be associated with inhibiting the expression of p53, and high-dose effect is good. [Keywords:] cerebral ischemia alprostadil P53 Cerebrovascular disease is harmful to human health, common diseases, including ischemic brain injury in possession of a large proportion of. Therefore, the development of new and effective treatment of cerebral ischemia is currently the research focus. This study attempts rats were given different doses of alprostadil pre-adaptation, application of immunohistochemical staining of apoptosis-related gene p53 were determined to explore alprostadil Preconditioning of the brain protection mechanisms provide a theoretical basis for clinical application . 1 Materials and methods 1.1 Experimental animals and grouping Healthy SD rats were 30 male and female in half, weight 200 ~ 250g, from the Experimental Animal Center of Jinzhou Medical College. Were randomly divided into sham operation group, ischemia group, alprostadil low dose group (1.25μ g / kg), the dose of alprostadil (2.5μ g / kg), and alprostadil high-dose group (5.0μ g / kg) ,