AMPK inhibition of HO-1 in cardiac hypertrophy.docVIP

 PAGE \* MERGEFORMAT 16 AMPK inhibition of HO-1 in cardiac hypertrophy Of: Chenguang Qin, Liu Chen and Zhang Chengxi, Meng Rong Sen, Chen Baolin, Xiongzhao Jun, Dong Calls [Abstract] [Objective] AMP-activated protein kinase (AMPK) and heme oxygenase HO-1 activation on myocardial hypertrophy, and HO-1 inhibition of cardiac hypertrophy in AMPK’s role. [Method] in cultured neonatal rat cardiac cells were studied by Western blot hybridization detection of 5 - amino -4 imidazole carboxamide ribonucleotide (AICAR) on AMPK phosphorylation and the extent of the impact of HO-1, and of myocardial cells [ 3H] - leucine incorporation and cell surface area was observed HO-1 AMPK and myocardial hypertrophy. [results] AICAR on AMPK and the role of HO-1 are activated, AICAR blocked the cardiac hypertrophy induced by Ang effect; blocking the activation of HO-1 is able to decrease the inhibitory effect of AICAR. [Conclusion] AMPK can inhibit cardiac hypertrophy, AMPK can activate HO-1, HO-1 in myocardial hypertrophy of the mechanism of AMPK plays an important role. [Keywords:] AMPK; HO-1; cardiac hypertrophy Abstract: [Objective] To investigate the effect of AMPK and HO-1 activation on hypertrophic cardiac myocyte, and the effect of HO-1 in the mechanism of AMPK inhibition of cardiomyocyte hypertrophy. [Methods] Cultured rat neonatal cardiacmyocytes were incubated with Ang to induce cardiac hypertrophy. Cardiac myocyte surface area and [3H] leucine incorporation was measured to determine the effect of AMPK and HO-1 on cardiac hypertrophy. The effect of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) on adenosine monophosphate activated protein kinase (AMPK) phosphorylation and HO-1 activaton were detected by Western blot. [Results] Compared with control group, activated AMPK significantly enhanced the transcriptive activity of AMPK, and increased HO-1 protein expression in cardiac myocyte. The increasement of cell surface area by Ang was significantly inhibi