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Automatic biochemical analyzer reagents reasonable sort
Author: Yang Yan, Rong-Tong, Cong Dong Zi
Keywords: sequencing reagents
【Abstract】 Objective To investigate the automatic biochemical analyzer reasonable sequencing reagents to improve detection accuracy. Approach end-point method, enzymatic determination results under different conditions comparison. Sort the results of reagent accuracy unreasonable influence the outcome. Conclusion The application of automatic biochemical analyzer, should pay attention to the rational arrangement of reagents in order to improve the accuracy of test results.
Keywords: sequencing reagents; error; accuracy
Automatic biochemical analyzer self-purification rate is limited, as the instrument to extend the life cycle and a variety of subjective and objective factors, an increase of reagent probe carrier rate of contamination, detection reagents sort properly, it may appear irreversible error, a direct impact on the reliability of test results, it is easy to cause clinical invalid data, a direct impact on the clinical diagnosis and treatment. Through long-term clinical test of practice and refer to the contents of the relevant literature, summarized in a clinical biochemical test sequence of reagents, so that multiple projects during the same test specimen when the result of reagents to minimize errors caused by mutual interference between the increase in the batch test results accuracy.
1 Materials and methods
1.1 The test specimens
(1) Subject to the review of serum: outpatient, inpatient intravenous serum; (2) quality control serum: Roche companies; (3) Standard solution: Roche Company, Beijing Lidman Biochemical Technology Co., Ltd. to provide.
1.2 Detection Kit
Roche original and Biochemical Technology Co., Ltd. Beijing Lidman finished kit.
1.3 instrumentation
Roche MODULAR 2p biochemical analyzer.
1.4 Det
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