Bacterial Identification System appears unable to identify bacteria treatment.docVIP

 PAGE \* MERGEFORMAT 6 Bacterial Identification System appears ‘unable to identify bacteria’ treatment We are now more general use of the VITEK-AMS identification system reported ‘can not identify bacteria’ (UIO) strains were further analyzed to explore the reasons for its occurrence, treatment methods, with a view to establish a set of practical instruments and manual methods of combining bacterial identification. One, materials and methods 1. Instruments and reagents: VITEK-AMS 60 automatic bacterial identification instrument and its supporting GNI, GPI, YBC test card (bioMé rieux products). 2. Test strains: 1 025 hospital 1999.10.21-2000.3.3 strains isolated from clinical samples were Vitek-AMS identified 631 cases in which application of GNI cards, GPI card 334 cases, YBC card 60 cases. 3.VITEK-AMS identification method: According to the phenotypic characteristics of bacteria, select the appropriate GNI, GPI, YBC card, in strict accordance with equipment operating instructions, incubation time, when the results of the report for the UIO device when the growth of the control picked holes (GPI Card No. 1 Kong, GNI card No. 3 hole, YBC card 24 holes) suspension in non-selective medium to re-separated to observe whether the pure culture, if, respectively, in accordance with the law and were identified by the following two. Otherwise, by the pure culture and re-identification on the machine. 4. Bifidobacterium retrieve identification method: According to the Vitek microbial analysis system for automatic technical information provided by Bifidobacterium keys, depending on the results of retrieval to biological genus or species. 5. Conventional identification method: In accordance with the national clinical laboratory operating procedures [1] carried out and approved by API systems (bioMerieux company) to review the identification of species. Some strains identified based on reference [2] carried out. Second, the results of 1.UIO incidence