Baicalin on human hepatoma cell line SMMC7721 Experimental Study of anti-tumor effect.docVIP

 PAGE \* MERGEFORMAT 15 Baicalin on human hepatoma cell line SMMC7721 Experimental Study of anti-tumor effect Author: state of the South FU Ying-yuan Huang Bin-Hong Zhou Zhen Xing Zeng Xiaoping [Abstract] Objective To study the baicalin on human hepatoma cell line SMMC7721 growth inhibition and to explore its mechanism. Methods MTT assay of baicalin on the proliferation of human liver cancer cell line SMMC7721 inhibition, using flow cytometry of its SMMC7721 cell cycle. Results ① 4 ~ 0.005 312 5 mg / ml of baicalin group SMMC 7721 growth of tumor cells were obviously inhibited, and cell group and the control over significant difference (P amp;lt;0.05 or P amp;lt;0.01). 0.5 ~ 0.062 5 mg / ml of baicalin group of tumor growth inhibition rate compared with the other concentration groups were significantly different (P amp;lt;0.01), and cisplatin control group, no significant difference (Pamp;gt; 0.05). When baicalin concentration of 0.5 mg / ml, its inhibition of tumor cell growth rate in the highest, 63.8%; ② 0.5 mg / ml of baicalin on the SMMC7721 damage of tumor cells form the most obvious, showing that 60% to 70% of the debris-like cells were a small number of cells were suspended growth; ③ 0.5 mg / ml of baicalin group able to stagnation in the G0/G1 phase cells (G0/G1 phase cells of 72.19%). Conclusion of baicalin on the growth of SMMC 7721 cells have a significant inhibitory effect, and may affect its cell cycle. [Keywords:] anti-tumor effect of baicalin Abstract: ObjectiveTo investigate the inhibitory effect of baicalin on the growth of human hepatic carcinoma cell line SMMC 7721 in vitro and discuss its mechanism.MethodsThe inhibitory fraction of SMMC 7721 cells was measured by MTT assay, and flow cytometry was used to determine the effect on cell cycle.Results ① . Baicalin in a concentration range of 4 ~ 0.005 312 5 mg / ml had remarkable inhibitory effect on the growth of SMMC 7721 in vitro, and had statistically significant difference in compari