Chromatography and hydrodynamic chromatography and its application barriers.docVIP

 PAGE \* MERGEFORMAT 25 Chromatography and hydrodynamic chromatography and its application barriers [Abstract] hydrodynamic chromatography (HDC and obstacles chromatography (SC and its biological, chemical separation of the research progress, focusing on their separation principle, theory development and the linkages between and transformation, citing 52 documents . [Keywords:] dynamic liquid chromatography, hydrodynamic chromatography, obstacles chromatography, DNA separation, review Abstract Hydrodynamic chromatography (HDC) and slalom chromatography (SC) called as dynamic liquid chromatography (DLC) were introduced and reviewed, mainly for the recent development of separation principle, theoretical model, and applications. Fifty two references were cited. Keywords Dynamic liquid chromatography, hydrodynamic chromatography, slalom chromatography, deoxyribonucleic acid separation, review 1 Introduction With the deepening of life science research, liquid chromatography (LC) in the Purification of the application of peptides and proteins has also been developing rapidly as possible to improve the LC separation has become the subject of one of the most important [ 1,2]. Over the years, studies of Retention behavior of the solute is essentially based on the thermodynamic factors, that is based on the components in the stationary phase and mobile phase to achieve differences in the distribution coefficient of the separation of components, and assume that components In the second phase of distribution or instantaneous adsorption equilibrium is completed. However, as early as 1956, Dutch scholar Van Deemter have proposed the kinetic theory of the chromatographic process - rate theory [3]. He will chromatographic process as a dynamic non-equilibrium process, the dynamics of the process of separation factors on the impact of peak broadening. Since then, Giddings et al [4] Van Deemter equation is proposed based on the LC rate equation, that is, G