CIK cell killing effect of curcumin on cell line SGC-7901 gastric cancer effects and mechanism of.doc

 PAGE \* MERGEFORMAT 14 CIK cell killing effect of curcumin on cell line SGC-7901 gastric cancer effects and mechanism of Of: Jiangyun Li 1, Su-Juan Fei, Chen rehabilitation, Liu Jun rights, Zhou Zhonghai [Abstract] Objective To study the effect of curcumin on cytokine-induced killer cells (CIK cells) anti-gastric cancer cell line SGC-7901 and its effects. METHODS 10 healthy peripheral blood mononuclear cells (PBMC) in vitro by a variety of Cytokine induction of CIK cells; collected 5 days of culture, CIK cells treated with different concentrations of curcumin induced, 37 , 5% CO2 and cultured under 72 h, LDH assay CIK cells SGC-7901 cell-killing activity ; FACS assay CIK cell phenotype CD3 + CD8 +, CD3 + CD56 + content and perforin, granzyme B levels. Results Curcumin induced, CIK cells against gastric cancer SGC-7901 activity was significantly increased in 10 mol / L when killing activity was significantly higher (P lt;0.05). curcumin induced, CIK cell perforin and granzyme B levels were significantly increased (P lt;0.05). curcumin could significantly increase the CIK cells in CD3 + CD56 + expression (P lt;0.05), reduce the CD3 + CD8 + expression (P lt;0.05). Conclusion curcumin can enhance the CIK cells against gastric cancer cell line SGC-7901 in the killing activity, which may be related to the increase CIK cells content of perforin and granzyme and increased CD3 + CD56 + expression. [Keywords:] curcumin; CIK cells; SGC7901 gastric cancer cells; killing activity; Perforin; granzyme B Abstract: Objective To investigate the effect and the underlying mechanism of the curcumin-induced cytotoxicity of CIK cells to gastric cancer cell line SGC-7901 in vitro. Methods The peripheral blood mononuclear cells (PBMC) from 10 healthy volunteers were induced in vitro with different cytokines and transferred into CIK cells. On the fifth day, the CIK cells were collected and were induced with the addition of curcumin at different concentrations, followed b