Critical value of anti-HIV preparation and application of quality control serum.docVIP

 PAGE \* MERGEFORMAT 7 Critical value of anti-HIV preparation and application of quality control serum [Abstract] Objective To investigate the critical value of anti-HIV laboratory quality control serum preparation and application of simple and practical method. Methods confirmed the high titer of anti-HIV positive sera by using bovine serum 1:1,1:2,1: 4,1:8,1:16,1:32,1:64 dilution OD values #8203;#8203;were measured to calculate the average (), standard deviation (s) and coefficient of variation (CV). the results of measured values #8203;#8203;of 20 S / CO value of the correlation value = 1.91, s = 0.155, CV = 8.1%. Conclusion The anti-HIV laboratory critical value of self-control serum can be used to monitor the stability of anti-HIV testing, repeatability, accuracy. [Keywords:] of anti-HIV quality control sample preparation Currently, ELISA is recognized as a good anti-HIV screening method [1], in the daily anti-HIV testing process, requiring a blank, yin and yang of the yin and yang of the control and the control value by a critical value (Cut off), according to The yin and yang of judging the results. But in practice, the detection of anti-HIV affected by many factors, the systematic error (including the kit quality issues, the accuracy of pipettes and microplate reader, water bath and ambient temperature fluctuations etc.) and random error (plus sample plus or misuse are not allowed to leak or even increase, etc.), resulting in a larger deviation of test results. Therefore, in the experiment and then add a control serum of the experimental critical value of dynamic control, to establish a more perfect quality control system is the key step to ensure the quality test. I made critical by the positive control sera sera, in practice, satisfactory results achieved. 1 Materials and methods 1.1 Materials 1.1.1 Reagents Anti-HIV ELISA kits, anti-HIV positive serum Biological Pharmaceutical Co., Ltd. from Beijing to provide Manhattan, batch nu