Curcumin inhibition of multiple myeloma cells brain-derived neurotrophic factor-induced angiogenesis.docVIP

 PAGE \* MERGEFORMAT 4 Curcumin inhibition of multiple myeloma cells brain-derived neurotrophic factor-induced angiogenesis [Abstract] To study the effect of curcumin on multiple myeloma (MM) cells, brain-derived neurotrophic factor (BDNF), endothelial cells and endothelial cells, the expression of TrkB angiogenesis effect of a preliminary study curcumin treatment by inhibiting angiogenesis the possibility of multiple myeloma, using RT-PCR curcumin were detected before and after treatment of multiple myeloma cell line KM3 in BDNF gene expression and endothelial cell line ECV304 in the TrkB gene expression changes in application testing and migration of endothelial cells endothelial cell tube formation testing, evaluation of curcumin on endothelial cell angiogenesis effects. The results showed that: Exogenous BDNF can effectively promote endothelial cell migration and tube formation, but the two effects can be markedly blocked curcumin; KM3 cells expressed BDNF mRNA, ECV304 cells expressed TrkB mRNA, effects of curcumin on the two inhibited the expression of both in a dose - time-dependent. Conclusion: BDNF is a kind of promotion of vascular proliferative activity of cytokines, curcumin can be lowered by MM cells, BDNF and TrkB expression in endothelial cells impede the interaction between the two, followed by inhibiting angiogenesis, which may be a potential treatment of MM role of the target. [Keywords:] curcumin Inhibitory Effect of Curcumin on Angiogenesis Induced by Brain Derived Neurotrophic Factor from Multiple Myeloma Cells Abstract In order to explore the probability of curcumin treating multiple myeloma (MM) via the inhibition of angiogenesis, the expressions of brain derived neurotrophic factor (BDNF) and its specific receptor in human MM cells and endothelial cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The angiogenic activity was evaluated by endothelial cell migration assay and tubule formation assay in vitro