Determination on Shuanghuanglian oral solution of chlorogenic acid baicalin phillyrin the content of wogonoside_0.docVIP

 PAGE \* MERGEFORMAT 14 Determination on Shuanghuanglian oral solution of chlorogenic acid baicalin phillyrin the content of wogonoside [Abstract]: Objective To establish the simultaneous determination of chlorogenic acid in SHL oral liquid, baicalin, phillyrin and wogonin HPLC method. Methods VP-ODS C18 column (250 mm × 4.6 mm, 5 μm), mobile phase: acetonitrile -0.2% phosphoric acid gradient elution, flow rate: 1.0 mL / min, detection wavelength: 278 nm. Results of chlorogenic acid in the range of 0.44 ~ 6.60 μg good linear relationship (r = 0.999 1) The average recovery was 97.0%, baicalin in the range of 0.52 ~ 6.18 μg good linear relationship (r = 0.999 1), the average recovery was 98.98%, forsythin in the range of 0.20 ~ 2.04 μg good linear relationship (r = 0.999 3), the average recovery was 103.55%, wogonin in the range of 0.13 ~ 1.76 μg good linear relationship (r = 0.999 1), the average recovery was 96.49%. Conclusion The method is simple, accurate, rapid and can be used for Shuanghuanglian Oral above 4 Determination of the contents. [Keywords:] Shuanghuanglian oral chlorogenic acid baicalin wogonin forsythin HPLC determination Abstract: Objective To establish a HPLC method for the determination of chlorogenic acid, baicaliin, forsythin and wogonin in Shuanghuanglian oral liquid. Methods The column was VP-ODS C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile -0.2% phosphoric acid with gradient elution. The flow rate was 1.0 mL / min and the detection wavelength was at 278 nm. Results The calibration curves were linear within the range of 0.44 ~ 6.60 μg (r = 0.999 1) for chlorogenic acid, 0.52 ~ 6.18 μg (r = 0.999 1) for baicaliin, 0.20 ~ 2.04 μg (r = 0.999 3) for forsythin (r = 0.999 3) and 0.13 ~ 1.76 μg (r = 0.999 1) for wogonin, respectively. The average recovery of them were 97.0% (RSD = 1.1%), 98.98% (RSD = 1.1%), 103.55% (RSD = 1.1%) and 96.49% (RSD = 1.1%), respectively. Conclusion The method is simple, practicab