Different species of Gynostemma isoenzyme spectrum of preliminary study.docVIP

 PAGE \* MERGEFORMAT 24 Different species of Gynostemma isoenzyme spectrum of preliminary study Author: Liu Jinlong TIAN Guo-zheng Zhao Dong-Fa Sun [Abstract] Objective Gynostemma medicine for the valuable edible Chinese herbs, variety traits similar to Analogues. Zymogram with the same power to establish production from the biochemical level of promotion of Jiaogulan germplasm files, for breeding of new varieties and purification of re-shaped to provide basis for germplasm identification. Methods SDS-polyacrylamide gel electrophoresis technique Gynostemma isoenzyme spectrum. The results en 5 leaf honey, en Aescin sweet, Gynostemma, the original variant of eight leaves of enzymes with a number of natural lines followed by 9,9,9,10; enzyme was followed by four deep-colored 5 shallow, two deep-7 shallow, 3 deep-six shallow, two deep-eight shallow; enzyme followed by width of 6.00 ~ 8.56 nm, 4.87 ~ 6.61 mm, 6.01 ~ 6.84 mm, 4.87 ~ 6.61 mm; enzyme was followed with a Rf value of 0.14 ~ 0.90,0.16 ~ 0.93,0.14 ~ 0.76,0.07 ~ 0.89. Conclusions The study found that their isozyme big difference, from the biochemical molecular level prove that they are different species, can be used as the basis Gynostemma germplasm identification. [Keywords:] different species of Gynostemma pentaphyllum; catalase isozyme Jiaogulan Gynostemma pentapHyllum (Thunb.) makino Department of Cucurbitaceae climbing herbaceous perennial climbing plant medicines for rare edible medicine. Which contains a variety of saponins, amino acids, polysaccharides, trace elements, such as phospholipid and flavonoid compounds, but also four kinds of gypenosides with the same structure as ginsenosides, there are 16 kinds of gypenosides obtained after hydrolysis of ginsenosides K, known as the ‘ second ginseng ‘. With anti-fatigue, anti-aging, anti-hypoxic effect, long-term use can enhance the human immune system, while there have been significant sedative, hypnotic, anti-fatigue, lowering blood pressure, nour