Differentially expressed proteins of hepatitis B liver fibrosis screening and functional study DDAH1.docVIP

 PAGE \* MERGEFORMAT 15 Differentially expressed proteins of hepatitis B liver fibrosis screening and functional study DDAH1 Authors: LUO Xin-hua, Cheng ML, Zhang Quan, Yang Qin [Abstract] Objective: To compare the analysis of hepatitis B fibrosis and non-fibrotic liver tissue proteome expression profile differences, explore dimethylarginine dimethylamino hydrolase 1 (DDAH1) in the occurrence and development of liver fibrosis functions. Methods: Using two-dimensional gel electrophoresis (2-DE) screening of liver fibrosis and liver fibrosis of non-differentially expressed proteins identified by mass spectrometry differences in protein, Western-blot verified expression levels of DDAH1; DDAH1 activity measured in liver tissue, asymmetric dimethylarginine (ADMA) levels, nitric oxide synthase (NOS) activity and NO2-/NO3- ratio. Results: A total of 25 identified DDAH1 more than 2-fold difference in protein; liver fibrosis DDAH1 the activity decreased, ADMA was increased, NOS activity declined, nitric oxide (NO) content decreases. Conclusion: Hepatitis B liver fibrosis with non-fibrotic liver tissue of protein expression profiling have certain differences, DDAH1 is possible by regulating the metabolism of ADMA affect NO synthesis, involved in the occurrence and development of hepatic fibrosis. [Keywords:] Hepatitis B, chronic; fibrosis; proteomics; 2-methyl-arginine; 2-methyl-amino hydrolases [Abstract] Objective: To compare and analyze the difference of proteomic expression profiles between human hepatitis B (HB) derived hepatic fibrosis tissues and non-fibrosis liver tissues, and analyze the functions of dimethyl aminohydrolase (DDAH1) in the formation and development of hepatic fibrosis. Methods: The differential expression of proteins were screened with two dimension electrophoresis (2-DE) and identified with mass spectrogram after the total protein was extracted from hepatic fibrosis tissue and nonfibrosis liver tissue. DDAH1 expression was verified with West