Dry Banxia Optimization of tissue culture conditions.docVIP

 PAGE \* MERGEFORMAT 8 Dry Banxia Optimization of tissue culture conditions Authors: Li-Yan Wang, Jing Rui Yong, Huan-Bo, Tang Min, Kui-Germany [Abstract] Objective To optimize the conditions for tissue culture of Pinellia ternata and droughts. Method to dry a small Pinellia tuber as explants for tissue culture. Results and Conclusion carrageenan instead of agar solidified, and achieved good results; dry Pinellia optimum subculture medium was: MS 6-BA 2.0 mg / L NAA 0.5 mg / L, the proliferation coefficient of 6.5, the average seedling Up to 2.5 cm; the optimal medium for rooting: 1/2MS NAA 0.1 mg / L, root number 4 ~ 5, rooting rate reached 100%. [Keywords:] dry ternata; tissue culture; carrageenan Abstract: Objective To optimize tissue culture condition of pinellia tenata (Thumb) Breit.Methods With the tuber of Pinellia tenata (Thumb.) Breit as explant, the culture condition of tissue culture was optimized by tissue culture.Results and Conclusion The effect of κ -Carrageenan replacing of agar is good.The suitable subculture Proliferation medium is MS 6-BA 2.0mg / L NAA 0.5 mg / L, the coefficient of proliferation get to 6.5, the average height is 2.5 cm, the suitable rooting medium is 1 / 2 MS NAA0.1 mg / L, the rooting count is about 4 ~ 5, and the ratio of rooting gets to 100%. Keywords:: Pinellia tenata (Thumb.) Breit; Tissue culture; κ -Carrageenan Pinellia pinellia tenata (Thunb.) Breit for Araceae perennial herb, with tubers medicine, is an important medicinal herb. Their sexual temperature, Weixin, a small drug, spleen, stomach, lung, through the post-cooked Runzao phlegm, Jiangni vomiting, Xiaopi Sanjie the effectiveness of [1]. Dry Banxia mostly wild, despite the obvious weed, but its reproduction coefficient low, but also the lack of effective means of communication, so it is a pioneering species poor [2]. In recent years, follows years of dredging, wild resources are depleting and far from being able to meet the market demand. Artificial cu