Enzyme assisted Extraction of polysaccharides of Elaeagnus angustifolia.docVIP

 PAGE \* MERGEFORMAT 12 Enzyme assisted Extraction of polysaccharides of Elaeagnus angustifolia On: Jun inexpensive, AUGUST FIRST LAND RECLAMATION Liu Hong, Mayan Mei, Chen Hanying [Abstract] Objective To study the complex enzyme assisted the optimal ultrasonic extraction process of polysaccharide Elaeagnus angustifolia. Methods cellulase and pectinase was investigated at different temperatures, different enzyme, under different time of enzyme solution extracted angustifolia polysaccharide, and of ultrasonic treatment on the enzymatic extraction of polysaccharides Elaeagnus angustifolia. Results angustifolia appropriate conditions Extraction: 1.5% cellulase, pectinase 1.5%, reaction time 40 min, hydrolysis temperature of 55 ℃ , ultrasonic time 25 min, ultrasonic power 400 W, solid-liquid ratio of 1:30, ultrasonic temperature 60 ℃. Under these conditions, the extraction rate of polysaccharides angustifolia 12.35%. Conclusion The technique is simple and feasible, can be used for Elaeagnus polysaccharide extraction. [Keywords:] Elaeagnus angustifolia; polysaccharides, enzymes, ultrasonic extraction Abstract: Objective To optimize the technological conditions of polysaccharide extraction from Elaeagnus angustifolia L. by enzymatic-assisted ultrasonic. Methods The effects of different enzymes, enzymolysis temperature, enzyme dosage, enzymolysis time, material-water ratio, crushing degree, ultrasonic time and ultrasonic temperature on the extraction rate of Elaeagnus angustifolia L. polysaccharide was analyzed. Results The optimal technological condition was as follows: 1.5% cellulase, 1.5% pectic enzyme, enzymolysis time 40 min, enzymolysis temperature 55 ℃, ultrasonic time 25 min, ultrasonic power 400 W, material-water ratio 1:30, and temperature 60 ℃. In these conditions, the extraction efficiency of polysaccharide was 12.35%. Conclusion The process is simple and practicable, and can be used for the extraction of Elaeagnus angustifolia L. polysacchar