Enzyme-linked immunosorbent assay detection of HBV anti-HBc false positive of.docVIP

 PAGE \* MERGEFORMAT 7 Enzyme-linked immunosorbent assay detection of HBV anti-HBc false positive of [Abstract] Objective To identify and correct the enzyme-linked immunosorbent assay (ELISA) detection of hepatitis B occurred in the course of anti-HBc false positive results. Method in hepatitis B ‘two pairs of semi’ clinical testing specimens proceeded to select anti-HBc-positive specimens were 310, according to ‘two pairs of semi-’ test results were divided into three groups of different models: A group of HBs-Ag (), anti - -HBs (-), HBeAg or anti-HBe (), anti-HBc (); B group of HBs-Ag (-), anti-HBs (), HBeAg (-), anti-Hbe (or -), anti HBc (); C group of other models. Specimens by high speed centrifugation (5000g, 10min), and then respectively Kehua and Emori the company’s reagents (both ELISA) review of anti-HBc. Results in 310 samples (A group of 153, B group 96, C group 61) using Section China’s reagent to detect anti-HBc positive samples were 241, with Emori’s reagent to detect anti-HBc-positive specimens of 249 copies, both in line with the rate of 94.8%. 57 specimens with two kinds of reagents, after review of anti-HBc were negative, including two copies of A group, B group 49, C group six copies. Of each group accounting for 1.3%, 51.0% and 9.8% respectively. Conclusion of competition law ELISA detection of anti-HBc, due to non-specific adsorption and operation of the reasons for false-positive results easy, especially for anti-HBs and anti-HBc-positive specimens should be reviewed at the same time anti-HBc. [Keywords:] enzyme-linked immunosorbent assay false-positive anti-HBc hepatitis B Serum markers of hepatitis B in recent years, multi-purpose enzyme-linked immunosorbent assay (ELISA) competition law (one step), because of method is simple, fast and well received. However, using this method for anti-HBc detection is often a false positive quantities [1]. We are 310 anti-HBc-positive specimens were reviewed, these are as follows. 1 Materials and