EP2 synaptic transmission in hippocampal dentate gyrus of the role of.docVIP

 PAGE \* MERGEFORMAT 12 EP2 synaptic transmission in hippocampal dentate gyrus of the role of [Abstract] Objective EP2 in mouse hippocampal synaptic transmission in the dentate gyrus and its mechanism. Methods extracellular recording technique was penetrating hippocampal slices fiber - granule cell synapses in the dentate gyrus field excitatory postsynaptic potentials (fEPSP). Results EP2 agonist Butaprost enhance synaptic transmission in dentate gyrus and the lower two-pulse ratio (paired-pulse ratio, PPR), this effect was blocked by EP2 antagonist AH6809. Forskolin alone or Forskolin + AH6809 can enhance the fEPSP slope. EP2 enhanced synaptic efficacy of the effect of the PKA, ERK, and IP3-mediated pathway. Conclusion multiple signal transduction pathways may be involved in EP2 activation-induced enhancement of synaptic transmission. [Keywords:] prostaglandin, cyclooxygenase -2 (COX-2), EP2, synaptic transmission, hippocampus ChinaABSTRACT: Objective To evaluate the role of EP2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results Butaprost, an agonist of EP2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP2 antagonist). Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. The Butaprost- induced responses were mediated via PKA, ERK and IP3 signal pathways. Conclusion Multiple signal pathways were involved in the EP2 activation-mediated enhancement of synaptic transmission. KEY WORDS: prostaglandin; cyclooxygenase-2 (COX-2); EP2; synaptic transmission; hippocampus Cyclooxygenase -2 (cyclooxygenase-2, COX-2) is the transformation of arachidonic acid (arachidonic acid, AA) as an unstable intermediate PGG2 rate-limiting enzyme [1-2]. COX-2 in excitability glutamater