Eukaryotic expression plasmid pIRES2eGFPAnnexin A2 Construction and expression in 293T cells.docVIP

 PAGE \* MERGEFORMAT 12 Eukaryotic expression plasmid pIRES2eGFPAnnexin A2 Construction and expression in 293T cells Of: Li, Yu Zhou Ying, Wang Ting Huang Hongliang, Shiwen Xia Wang Haibo [Abstract] Objective: To construct containing human Annexin A2 gene eukaryotic expression plasmid pIRES2 eGFP Annexin A2, and expressed in eukaryotic cells 293T. METHODS: The plasmid pCMV5 Annexin A2 Annexin A2 gene digested with the eukaryotic expression plasmid pIRES2 eGFP connection, restriction enzyme digestion and sequencing, the liposome-mediated transfection of 293T cells, fluorescence quantitative PCR and Western blotting to detect the gene and protein expression. Results: The plasmid pIRES2 eGFP Annexin A2 transfected 293T cells , the target gene mRNA and protein were significantly increased. CONCLUSION: The constructed plasmid pIRES2 eGFP Annexin A2 and protein for the study of biological functions of Annexin A2 basis. [Keywords:] Annexin A2, eukaryotic expression, anti-phospholipid syndrome [Abstract] Objective: To construct pIRES2 eGFP Annexin A2 eukaryotic expression vector carrying human Annexin A2 gene and express Annexin A2 protein in 293T cell.Methods: Annexin A2 gene digested from pCMV5 Annexin A2 was ligated into eukaryotic expression vector pIRES2 eGFP.After restriction analysis and sequencing, the recombinant plasmid pIRES2 eGFP Annexin A2 was transfected into 293T cells in the mediation of liposome.The expression of Annexin A2 was analyzed by western blot and real time PCR. Results: The expression of Annexin A2 at both mRNA and protein levels were significantly increased when transfected with pIRES2 eGFP Annexin A2 in 293T cells. Conclusion: The eukaryotic expression vector pIRES2 eGFP Annexin A2 was correctly constructed and the Annexin A2 protein was successfully expressed in 293T cells.This will facilitate the following study on Annexin A2. [Keywords:] Annexin A2; eukaryotic expression, antiphospholipid syndrome Annexins are a class o