Establishment of rat model of metabolic syndrome.docVIP

 PAGE \* MERGEFORMAT 4 Establishment of rat model of metabolic syndrome [Keywords:] Metabolic Syndrome Metabolic syndrome (metabolic syndrome, MS) is a process involving a variety of metabolic abnormalities, cardiovascular disease closely associated with the disease state, of which obesity (overweight), blood pressure, blood glucose and lipid abnormalities 4 to highlight, MS patients with DM risk increased five-fold, CVD risk increased three-fold increased cardiovascular mortality two-fold, 1.5-fold increased risk of total mortality [1]. Therefore, the study of metabolic syndrome prevention and treatment in reducing the mortality rate of these diseases is very important, while the experimental animal model of the work is an important prerequisite for the study. An experimental material 1.1 The choice of laboratory animal health from the milk of Sprague-Dawley (SD) rats were 100 male and female in half, weighing 49 ~ 70g, regular clean grade, were purchased from Shandong Medical University Experimental Animal Center, Animal Production License No.: SCXK ( Lu) 20,050,015. 1.2 animal feed-based feed for Shandong Medical University Experimental Animal Center provides general clean grade animal feed, high fat high calorie diet with reference literature [2,3] improved formula as follows: normal diet 56%, lard 10%, sugar 10% , milk powder 10%, 12% egg yolk, soy flour 2%. Sugar, Shandong Province, Eastern Sugar Co., Ltd. for the production of superior grade cotton days park brand sugar, batch number for the GB1445, powdered milk for the Inner Mongolia Yili Industrial Group Co., Ltd. production of full cream sweet milk powder, lot number 81211F3, lard, egg yolk, soybean meal by the market to buy. Two kinds of feed composition and nutrient content in Table 1. 1.3 Main reagents and instruments Olypus Au2700 automatic biochemical analyzer (Toshiba production), Olympus Diagnostic Products Co., Ltd. to provide blood glucose, triglycerides, total cholesterol, high densit